摘要
目的研究慢性乙型肝炎(CHB)患者聚乙二醇干扰素和阿德福韦酯抗病毒治疗前后外周血树突状细胞(DC)的变化情况。方法慢性乙肝患者35例,其中聚乙二醇干扰素α-2a(40 kD)(派罗欣)治疗组18例,阿德福韦酯(贺维力)治疗组17例,采集治疗前、治疗12、24和48周时患者的外周血单个核细胞(PBMC),采用流式细胞技术检测mDC和pDC的百分比,并分析其与ALT和HBV-DNA的相关性。结果慢性乙肝干扰素组和阿德福韦酯组在治疗前mDC和pDC的比例和绝对计数均显著低于正常对照组(P<0.01)。抗病毒治疗后两组的mDC和pDC的比例和绝对计数均较治疗前有所增多,其中干扰素治疗组在治疗12周、24周和48周时mDC和pDC的的比例、数量与治疗前相比,均有显著提高(P<0.05)。而阿德福韦酯治疗组的mDC与治疗前相比差异无显著性,pDC的数目在治疗24周和48周时较治疗前显著升高(P<0.05)。mDC、pDC与ALT和HBV-DNA无明显的相关性。结论 CHB患者外周血mDC和pDC细胞降低,聚乙二醇干扰素和阿德福韦酯抗病毒治疗能改善患者的免疫功能状态,增加DCs的比例和数量。
【Objective】To investigate the characteristics of circulating dendritic cell subsets（including mDC and pDC） in chronic hepatitis B（CHB） patients accepting antiviral therapy. 【Methods】Peripheral blood mononuclear cells（PBMC） of 35 patients with CHB were collected at baseline and week 12, week 24 and week 48 treatment. Eighteen patients were treated with peginterferon alfa-2a（40 kD）（PEGASYS） and 17 patients were treated with adefovir dipivoxil（ADV）. The proportion of DCs was analyzed using flow cytometry. The correlation between DCs and ALT or HBV-DNA was also analyzed. 【Results】The proportion and absolute number of peripheral blood mDC and pDC in PEGASYS-treatment group and ADV-treatment group were significantly decreased comparing with those in health controls（P 0.01）. DCs levels were restored by antiviral therapy. In PEGASYS group, the proportions and absolute numbers of mDC and pDC at week 12, week 24 and week 48 treatment were significantly increased than that of baseline（P 0.05）. No statistically significant changes of mDC were found in ADV-treatment group. While the numbers and percentages of pDC at 24-week and 48-week treatment were significantly increased than that of baseline. No correlations were found between the increase of DCs and the decrease of HBV-DNA and ALT. 【Conclusions】The levels of peripheral blood mDC and pDC in CHB patients were significantly lower than those in health controls. PEGASYS and ADV antiviral treatment could restore the circulating DCs.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第4期38-41,共4页
China Journal of Modern Medicine
