摘要
以酶活和相对酶活为指标考察了极低频交变磁场对过氧化氢酶活性的影响。结果表明:不同磁场强度、频率及酶液pH、处理温度都会造成过氧化氢酶的活性变化,其变化趋势是随着磁场处理时间的延长,总体上其酶活和相对酶活降低。但就温度而言,当处理温度55℃下,相对酶活远高于100%,4 h达到最高值2 142.86%。圆二色谱测定表明,25℃磁场处理后酶的α-螺旋和β-折叠的总量小于对照组,55℃磁场处理后酶的α-螺旋和β-折叠的总量大于对照组,可推测过氧化氢酶在此条件下形成具有相对耐高温的结构。
In this manuscript,effect of the extremely low frequency alternating magnetic field on the specific and relative activity of catalase was investigated.The results suggested that magnetic field intensity,frequency and pH would generally decrease the specific and relative activity with the treatment time.But temperature changed specific and relative activity in a different way.The relative activity increased dramatically in the temperature of 55 ℃,reaching 2 142.86% at the time of 4 hours which was the highest point.According to circular dichroism（CD） results,the number of α helix andβ fold in 25 ℃ magnetic field treatment was less than the control group,but the result was opposite in 55 ℃,which indicated that catalase might form a relatively resistant to high temperature structure.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2014年第3期268-274,共7页
Journal of Food Science and Biotechnology
基金
国家"十二五"科技支撑计划项目(2012BAD37B06)
关键词
极低频交变磁场
过氧化氢酶
酶活
圆二色谱
extremely low frequency alternating magnetic field
catalase
enzyme activity
circular dichroism（CD）
