摘要
近似生理条件下,采用共振光散射法研究了CdSe/ZnS量子点/TiO_2纳米复合物同人血清白蛋白的相互作用,通过分析影响二者相互作用的纳米复合物浓度、pH、NaCl浓度、反应温度、检测时间、共存离子、表面活性剂、加样顺序等外源刺激因素,结果表明:新形成的复合体系可能加强蛋白质的疏水腔,使在水溶液中形成的疏水界面趋于集中,从而导致其共振光散射强度增强;体系受pH的影响变化非常灵敏;适当的NaCl浓度可以提高体系的I_(RLS)值灵敏度;共存离子改变了体系的离子强度,从而导致体系的△I_(RLS)值的改变;反应时间为5 min时,体系I_(RLS)值基本稳定;同一种表面活性剂对于不同的体系的I_(RLS)值的作用不完全一致,带相反电荷的表面活性剂与纳米复合物有很强的静电作用;对于与HSA相互作用的多元复合体系,加样顺序的不同对体系的I_(RLS)值的影响很明显;体系的I_(RLS)值随温度的改变呈不完全单调增加的趋势。这些信息为纳米材料与生物大分子的相互作用机制提供理论支撑,有助于深入了解纳米材料的生物相容性和安全性。
The interaction between CdSe/ZnS（quantum dots）/TiO2 nanocomposites and human serum albumin（HSA） was inves-tigated by resonance light scattering （RLS） spectroscopic methods under approximate physiological conditions .Much important information of the interaction between CdSe/ZnS（Quantum Dots）/TiO2 nanocomposites and HSA was obtained by studying com-prehensively the Exogenous influence factors of nanocomposites concentration ,pH ,NaCl concentration ,reaction temperature , detection time ,coexisting ions ,surfactants ,sequence of adding to the sample etc .It was showed that the new formation of com-plex system is likely to enhance the protein hydrophobic cavity and tend to focus the hydrophobic interface in aqueous solution , resulting in strengthening the intensity of resonance light scattering ;Also it is very sensitive to the changes in the pH value in the system ;The sensitivity of IRLS in system can be increased by the appropriate NaCl concentration ;The value of IRLS in sys-tem would be changed with the change in the concentration of coexisting ions ;The value of IRLS in system is basically stable when the reaction time reaches 5 min;The value of IRLS in system is not exactly the same with a surfactant ,and strong electrostatic in-teraction has occurred between oppositely charged surfactant and nano composites ;It is obvious that the value of IRLS in complex system is affected by the sequence of adding to sample ;It has the incomplete monotonically increasing trend with the changes in temperature .The information is useful for providing theoretical supporting for the mechanism of interaction between nanomateri-als and bio-macromolecule ,and for understanding the biocompatibility and safety of nano-materials .
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2014年第4期1040-1044,共5页
Spectroscopy and Spectral Analysis
基金
国家科技部(973计划)项目(2009CB939705)
国家自然科学基金项目(21225313)
教育部-民委共建湖北省催化材料重点实验室项目(CHCL09007)
国家级大学生创业训练和创业实践项目(201210927033
201310927023)
湖北科技学院专项基金项目(ZX1022
ZX1102
PY1103
BK1103
ZX1203)资助
