摘要
通过比较棉纤维织物、聚乙烯醇缩甲醛柱体、活性炭颗粒在35℃、厌氧条件下吸附丁酸梭菌(Clostridium butyricum)将甘油转化为1,3-丙二醇的有效性,选择活性炭颗粒作为固定化丁酸梭菌的较佳载体.以活性炭颗粒作为吸附载体时,初始甘油质量浓度从20g/L提高至252g/L,最终1,3-丙二醇质量浓度从9.5g/L增加至38.3g/L,同时残余甘油质量浓度从0g/L提高至96.3g/L,1,3-丙二醇的时空产率从0.7g/(L·h)提高至2.2g/(L·h),摩尔得率从65%降低至58%.
Clostridium butyricum was immobilized on the cotton fiber,polyvinyl alcohol column and activated carbon granule to produce 1,3-propanediol from glycerol.Fermentation in continuous culture was carried out in a fixed-bed bioreactor.Results showed that the activated carbon granule has desirable properties,such as biomass enrichment on the granule and operation convenience for continuous culture.When glycerol continuous loading rate raised from 0.24g/(L·h)to 3.00g/(L·h),the productivity of 1,3-propanediol raised from 0.7g/(L·h)to 2.2g/(L·h),the residual concentration of 1,3-propanediol raised from 9.5g/L to 38.3g/L,the yield of 1,3-propanediol reduced from 65%to 58%.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第2期227-232,共6页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(21076172)
福建省高校产学合作科技重大项目(2010H6023)
关键词
甘油
1
3-丙二醇
填充床
固定化细胞
发酵
glycerol
1,3-propanediol
packed-bed
immobilized cell
fermentation
