摘要
采用CTAB法对采集于贺兰山地区的两株野生大型真菌子实体进行基因组DNA提取,并利用真菌特有引物ITS1F和ITS4,在PCR基础上对其rDNA的ITS区段进行碱基序列测定,并与GenBank数据库中已知的相关序列进行Blast比对,结果表明,两株野生大型真菌分别属于鳞伞属(Pholiota sp.)和球盖菇属(Stropharia sp.),GenBank登录号分别为FJ596817.1和JQ627593.1。同时在UNITE Database(s)数据库中采用邻接法对两菌株进行系统发育树的构建及遗传距离的计算,结果表明,一菌株与Pholiota tuberculosa(Pers.)Kuyper&Tjall.-Beuk(UDB015324),另一菌株与Stropharia coronilla(Fr.ex Bull.)Quél.(UDB015429及UDB015556)的遗传关系最近,遗传距离达到5.0。初步鉴定两株野生大型真菌为小瘤鳞伞和齿环球盖菇。
The extraction of genomic DNA of two wild macrofungi obtained from Wulashan was carried out by CTAB method. Then, on the basis of PCR, ITS regions of DNA of the macrofungi were amplifiedby using the special pairs of primers ITS 1 F/ITS4 and sequenced. The sequences were compared with those registered in GenBank database, results of which showed that two fungi belonged to the genus Pholiota sp. and Strophariaceae sp .. Genbank accession numbers of the ITS gene was FJ596817.1 and JQ627593.1. Then the phylogenetic trees were constructed by neighbor-joining method and genetic distance was analyzed in UNITE Database （s）. The results showed that two wild fungi had a high genetic distance, one with Pholiota tuberculosa （Pers.） Kuyper ＆ Tjall.-Beuk （UDB015324）, and the other with Strop haria coronilla （Fr. ex Bull.） Quel.（UDB015429, UDB015556）, which was up to 5.0. On the basis of the sequence analysis, two wild fruiting bodyforming fungi were identified as PhoZiota tuberculosa and Strop haria coronilla.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第2期153-156,共4页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31260132
31060111)
内蒙古自然科学基金(2012MS0514)
关键词
野生真菌
分离
ITS
系统地位
wild fruiting body-forming fungi
isolation
ITS
phylogenetic position
