摘要
目的探讨正源方对环磷酰胺(CTX)致豚鼠骨髓抑制的保护作用。方法将豚鼠随机分为对照组、模型组、rhG-CSF组(20μg·kg-1)、正源方低、中、高剂量组(2.00,4.00,8.00g·kg-1)。正源方各给药组及rhG-CSF组在造模前提前给药3d,其余组给予同等剂量的生理盐水,连续给药18d。除对照组外,实验第4天各组肌肉注射CTX(64mg·kg-1),连续3d。于造模后第8天观察外周血象。实验结束后测定心、肝、脾、肺、肾等脏器指数,在显微镜下观察骨髓涂片的变化。结果与对照组比较,模型组豚鼠的外周血白细胞、红细胞、淋巴细胞、中性粒细胞计数、血红蛋白含量均不同程度地降低(P<0.01或P<0.05),骨髓涂片显示,骨髓巨核细胞受到不同程度地破坏;与模型组比较,各给药组的白细胞计数均显著上升(P<0.01),以rhG-CSF组提升白细胞、中性粒细胞计数最为显著(P<0.01);其中正源方中剂量组的白细胞、红细胞、淋巴细胞、中性粒细胞计数、血红蛋白含量均显著上升(P<0.01或P<0.05);各给药组豚鼠的心、肝、脾、肺、肾指数并无显著变化(P>0.05);骨髓涂片显示,正源方中剂量组对环磷酰胺抑制的骨髓有核细胞和巨核细胞系统修复作用最好。结论正源方能够促进环磷酰胺所致豚鼠骨髓抑制外周血的恢复和骨髓细胞的再生,对豚鼠的骨髓造血系统有一定的保护作用。
Objective To investigate the effects of Zhengyuan Prescription on the myelosuppression of guinea pigs induced by cyclo-phosphamide（CTX） .Methods Guinea pigs were randomly divided into control group ,model group ,rhG-CSF group （20 μg ＆#183; kg -1 ） ,and three groups each containing low （2 .00 g ＆#183; kg -1 ） ,medium（4 .00 g ＆#183; kg -1 ） ,high（8 .00 g ＆#183; kg -1 ） dose of Zhengyuan Prescription .Zhengyuan Prescription groups and rhG-CSF group were administered for 3 days before modeling ,while the remai-ning groups received physiological saline with equal volumes ,and the duration of the experiment was 18 days .Except for the con-trol group ,the other guinea pigs were induced by CTX （64 mg ＆#183; kg -1 ,im ,for 3 days） on the 4th day .Peripheral blood was ob-served at the 8th day after modeling .After the experiment ,the organ indexes of heart ,liver ,spleen ,lung ,and kidney were meas-ured and the changes of bone marrow smears were observed under the microscope .Results Compared with the control group ,the number of peripheral white blood cell（WBC） ,red blood cell（RBC） ,lymphocyte ,neutrophil and hemoglobin content of the model group decreased significantly （P＆lt;0 .01 or P＆lt;0 .05） .Furthermore ,the results of bone marrow smear revealed that bone marrow megakaryocytes were damaged in varying degrees .Compared with the model group ,WBC counts of the treatment groups were in-creased significantly（P＆lt;0 .01） ,especially for the group of rhG-CSF ,the WBC and neutrophil counts were enhanced most remark-ably（P＆lt;0 .01） .The blood cells counts of guinea pigs in the group with medium dose of Zhengyuan Prescription were increased significantly（P＆lt;0 .01 or P＆lt;0 .05） ,while the organ indexes of heart ,liver ,spleen ,lung ,and kidney had no significant changes（P＆gt;0 .05） .The results of bone marrow smear showed that the group of Zhengyuan Prescription with medium dose had a obvious therapeutic effect on the bone marrow nucleated cells and megakaryocytic cells system which was suppressed by CTX .Conclusion The recovery of peripheral blood and regeneration of bone marrow cells in myelosuppressed guinea pigs induced by CTX can be promoted by Zhengyuan Prescription which had a protective effect on the bone marrow hematopoietic system .
出处
《西北药学杂志》
CAS
2014年第2期148-152,共5页
Northwest Pharmaceutical Journal
基金
国家十二五"重大新药创制"科技重大专项(编号:2011ZX09401-308-037)
陕西省教育厅产化项目(编号:08JC16)
陕西省重点科技创新团队计划项目(编号:2012KCT-20)
关键词
正源方
环磷酰胺
白细胞
骨髓抑制
Zhengyuan Prescription
cyclophosphamide
white blood cell
myelosuppression
