摘要
目的:构建酵母细胞表面展示载体。方法:通过酶切连接方法在pADH1载体中插入信号肽、α-凝集素(含连接子)编码序列构建表面展示载体,并用EGFP来验证该载体的功能。结果:得到了267 bp的信号肽序列、1 623 bp的凝集素编码序列和717 bp的绿色荧光蛋白编码序列,酵母细胞表面展示载体被成功构建,且绿色荧光蛋白被插入到pADH1-agg中后,阳性转化子能在荧光显微镜下呈现绿色荧光。结论:这说明酵母细胞表面展示载体已经构建成功,并能成功表达和展示蛋白在酵母细胞表面。该载体的构建成功将为利用酵母表面展示系统表达和展示相关蛋白提供了平台。
Objective：Construct a yeast cell surface display vector. Method：The signal peptide and α -agglutinin encoding sequence will be inserted into the pADH1 by enzyme digestion and enzyme ligation. Result：267bp signal peptide, 1 623 bp α -agglutinin and 717 bp EGFP encoding sequence were amplified, and the yeast cell surface display vector was constructed successfully. The EGFP can be ex- pressed and displayed on the surface of yeast cell. Conclusion： The yeast cell surface display vector was constructed successfully. The vec- tor will provide a tool to express and display interesting protein on the yeast cell surface.
出处
《生物技术》
CAS
CSCD
北大核心
2014年第1期1-4,共4页
Biotechnology
基金
高等学校博士学科点科研专项基金项目(No.200805041071)
国家自然科学基金项目(No.31071588)资助
