摘要
目的 通过分子生物学与常规免疫组化法比较 ,以寻求更好地检测乳腺癌微小转移的方法。方法 采用逆转录多聚酶链反应 (RT PCR)方法检测 2 6例乳腺癌患者的外周血与骨髓中细胞角蛋白 19(KT19)mRNA的表达 ,并用链酶亲和素 生物素 过氧化物酶复合物 (SABC)免疫组化法检测乳腺癌患者骨髓涂片中上皮膜抗原 (EMA)。结果 2 6例外周血中KT19mRNA阳性 4例(15 4% ) ,骨髓阳性 10例 (38 4% )。免疫组化结果显示 2 6例骨髓中有 7例 (2 6 9% )EMA阳性 ,其KT19mRNA都阳性 ,有 3例 (11 5 % )免疫组化结果阴性而KT19mRNA阳性。结论 RT PCR方法检测KT19mRNA是一种比较敏感的方法 ,免疫组化也是一种比较可靠的检测方法 。
Objective In order to find a more sensitive method to detect micrometastases of breast cancer.Methods We applied the highly sensitive reverse transcriptase polymerase chain reaction (RT PCR)technique to study keratin 19 mRNA expression in the bone marrow and peripheral blood of 26 patients with primary breast cancer.These results were compared with that of immunohistochemistry,which could detect the expression of epithelial membrane antigen(EMA)on the breast cancer cells.Results 4 cases (15 4%)in blood and 10 cases (38 4%)in bone marrow were positive for the expression of keratin 19 mRNA.In all 10 bone marrow positive cases,3 cases were found to express keratin19 both in blood and in bone marrow and 7 cases were negative for keratin 19 mRNA in blood.There was one case in 4 blood positive cases which bone marrow was negative for keratin 19 mRNA expression.Compared with the results of immunohistochemistry,7(26 9%) cases were EMA positive which all were positive for keratin 19 mRNA.3 cases indicating the presence of micrometastases which could be detected by RT PCR but not by histological examination.Conclutions These results demonstrate that RT PCR is more sensitive than histological examination,but the latter is still a reliable method to be used in the clinical examination.
出处
《江苏医药》
CAS
CSCD
北大核心
2001年第2期93-95,共3页
Jiangsu Medical Journal
