摘要
目的：建立滇牡丹中没食子酸和丹皮酚含量测定方法,并比较滇牡丹不同产地及其不同部位中没食子酸和丹皮酚的含量.方法：采用ZORBOX SB-C18（4.6 mm×250 mm,5μm）色谱柱,以乙腈-0.1％甲酸溶液为流动相,梯度洗脱,流速1.0 mL· min-1,检测波长275 nm,柱温30℃.结果：没食子酸和丹皮酚在0.204 0～2.040 μg和0.204 0 ～2.040 μg呈良好的线性关系,平均加样回收率（n=6）为101.81％,97.80％.结论：该方法可用于滇牡丹药材的质量控制;不同产地滇牡丹中没食子酸和丹皮酚的含量差异较大.
Objective：To establish determined method of the content of trihydroxybenzoic acid and paeonol in Paeonia delavayi,and compare the content in samples from different areas and samples from different parts.Method：The samples were separated by a ZORBOX SB-C18 （4.6 mm × 250 mm,5 μm） using acetonitrile-0.1％ methane acid with water gradient system as mobile phase.The flow rate was 1.0 mL ·min-1,and the detection wavelength for fingerprinting was 275 nm with 30 ℃ for column temperature.Result：There were well linear relationships for trihydroxybenzoic acid and paeonol in range of 0.204 0-2.040 μg and 0.204 0-2.040 μg.And average recovery rates for them （n =6） were 101.81％ and 97.80％.Conclusion：The method could be used for quality control to medicinal materials of P.delavayi.Contents of trihydroxybenzoic acid and paeonol were significantly different for medicinal materials collected from different areas.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第5期57-60,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(31200180)
