摘要
采用聚丙烯酰胺梯度凝胶电泳技术 ,对合浦珠母贝 4种组织 (肝脏、外套膜、闭壳肌、鳃 )中的超氧物岐化酶 ( SOD)电泳图谱进行分析 ,结果表明 :SOD具有明显的组织特异性。在肝脏中 SOD由 3个位点编码 ,其中 Sod- 2、Sod- 3座位具有多态性 ;外套膜中 SOD由 3个位点编码 ,其中 Sod- 2、Sod- 4座位具有多态性 ;闭壳肌中 SOD由 4个座位编码 ,其中 Sod- 1、Sod- 2、Sod- 4座位具有多态性 ,肝脏 Sod- 3座位及闭壳肌 Sod- 1座位分别具有一“0”等位基因 ;鳃中 SOD至少由 7个以上位点编码 ,并出现三等位基因编码的
SOD isozyme in four tissues (liver,mantle,muscle and gill) of pinctada fucata were analysed by gradient gel electrophoresis on polyacrytamide.Results showed that SOD isozyme exhibited a tissue specificity apparently.There were three loci in liver and mantle respectively,Sod 2,Sod 3 showed polymorphisms in liver and Sod 2 ,Sod 4 in mantle;There were four loci in muscle and three loci had polymorphisms except Sod 3,Sod 3 in liver and Sod 1 in muscle had a '0' allele respectively;At least seven loci existed and a typical banding pattern SOD isozyme with three alleles was expressed in gill.
出处
《湛江海洋大学学报》
CAS
2000年第4期5-8,共4页
Journal of Zhanjiang Ocean University
