摘要
Ethyl methane-sulfonate (EMS)-mutagenized Arabidopsis M-2 populations were screened in low-K+ medium using the root-bending assay. Forty-two putative low-k(+)-tolerant (lkt) mutants were selected from 150 000 tested M-2 seedlings, and two of these mutants maintained their low-K+-tolerant phenotype in their M-3 generations, respectively. Genetic analysis showed that either one of these two mutants has a monogenic recessive mutation in a nuclear gene, and that the two mutations in two independent mutants are allelic to each other.
利用乙酰甲基磺酸 (EMS)诱变方法 ,以幼苗根在重力作用下的弯曲生长为指标 ,筛选得到了拟南芥 (Ara bidopsisthaliana)耐低钾突变体。经过对突变体杂交后代的遗传分析证明 ,其中两株突变体的耐低钾性状为隐性单基因突变所致。鉴定。
基金
SupportedbytheNationalNaturalScienceFoundationofChina ( 3 95 70 0 74
3 95 2 5 0 0 3
and 3 9873 95 6toWWH)andBeijingScienceFou
