摘要
以云南省保存的鲁桑、白桑、广东桑、鸡桑、长果桑、长穗桑6个桑种的33份种质资源为材料，采用Al（NO3）3-NaNO2比色法测定桑叶和桑枝中的总黄酮含量，筛选药食用途桑树品种的育种材料。33份桑树种质资源的桑叶总黄酮含量总体上符合正态分布，93．94％的检测样品的桑叶总黄酮质量比分布在10．00～35．00rag／g之间，96．97％的检测样品的桑枝总黄酮质量比分布在2．00—15．00mg／g之间，大多数桑树种质资源的桑枝总黄酮含量低于桑叶总黄酮含量。不同桑树种质资源间的桑叶、桑枝的总黄酮含量差异显著（P〈0．05）；但基于6个桑种间以及地方品种和选育品种之间的比较则没有显著差异（P〉0．05）。供试的33份桑树种质资源中，白桑种地方品种苍溪桑的桑叶总黄酮含量最高，质量比为35．27mg／g，长穗桑种地方品种普洱8的桑枝总黄酮含量最高，质量比为19．49mg／g，这2个地方品种适合作为育种素材用于培育药食用途的桑树新品种。
In this study, the contents of total flavonoids in leaves and branches of 33 mulberry germplasm resources from 6 mulberry species, namely Morus multicaulis Perr., Morus alba Linn., Morus atropurpurea Roxb., Morus australis Poir, Morus laevigata Wall., Morus wittiorum Hand-Mazz, in sericultural areas of Yunnan Province were determined by AI （ NO3 ） 3-NaNO2 colorimetry to screen breeding materials of mulberry varieties suitable for development of medicinal and food products. The results indicated that the contents of total flavonoids in leaves of 33 mulberry germplasm resources displayed normal distribution as a whole. Mass ratio of total fiavonoids in mulberry leaves of 93.94% test samples was distributed from 10.00 to 35.00 mg/g, that of 96.97% test samples was distributed from 2.00 to 15.00 mg/g. In most mulberry germplasm resources, the contents of total flavonoids in branches were lower than those in leaves. There was significant difference in total flavonoids contents from leaves and branches between various mulberry germplasm resources （P〈0.05）, while there was no significant difference between the 6 mulberry species and between local varieties and artificial bred varieties （P〉0.05）. Leaves of Cangxisang from M, alba Linn. had the highest levels of total fiavonoids, with mass ratio as high as 35.27 mg/g. Meanwhile, the mass ratio of total flavonoids in branches of Puer 8 from M. wittiorum Hand-Mazz had the highest level ot total flavnoids, with mass ratio as high as 19, 49 mg/g. These two local varieties could be used as breeding materials for new mulberry varieties cultivation for development of medicinal and food products.
出处
《蚕业科学》
CAS
CSCD
北大核心
2014年第1期85-90,共6页
ACTA SERICOLOGICA SINICA
基金
云南省现代农业蚕桑产业技术体系建设专项(No.2013KJTX-006)
现代农业产业技术体系专项(No.CARS-22)
商务部茧丝绸发展专项
关键词
桑树种质资源
桑叶
桑枝
总黄酮
云南蚕区
Mulberry germplasm resource
Mulberry leaf
Mulberry branch
Total fiavonoid
Yunnan sericultural area
