摘要
背景:多项实验表明牙胚组织在不同的时间点有不同的基因发挥作用,共同促进牙胚发育。目的:观察牙本质基质蛋白1、成釉蛋白、Ⅰ型胶原蛋白和同源异型盒基因1在大鼠牙胚细胞体外培养的不同时间的表达。方法:对体外培养后第1,3,6天的牙胚细胞提取RNA,反转录后采用实时定量PCR的方法检测牙本质基质蛋白1、成釉蛋白、Ⅰ型胶原蛋白和同源异型盒基因1mRNA相对表达水平的变化。结果与结论:牙胚细胞中牙本质基质蛋白1、成釉蛋白和Ⅰ型胶原蛋白mRNA的表达随培养时间的延长而增加,在培养3 d时达到峰值(P<0.05),而同源异型盒基因1 mRNA的表达随培养时间的延长而下降(P<0.05)。
BACKGROUND： Some studies have indicated that different genes in tooth germ tissue play a role at different time, contributing to tooth germ development.
OBJECTIVE： To observe the expressions of dentin matrix protein 1, enamel protein, collagen I and homeobox gene 1 at different stage of in vitro culture of tooth germ cells.
METHODS： RNA from tooth germ cells was extracted at days 1, 3, 6 of in vitro culture. After reverse transcription, real-time quantitative PCR detection was adopted to measure relative expression of dentin matrix protein 1, enamel protein, collagen I and homeobox gene 1 mRNA.
RESULTS AND CONCLUSION： Dentin matrix protein 1, enamel protein, and collagen I mRNA expressions increased with culture time, and reached the peak at day 3 （P 〈 0.05）, whereas homeobox gene 1 mRNA decreased with culture time （P 〈 0.05）.
出处
《中国组织工程研究》
CAS
CSCD
2014年第2期193-198,共6页
Chinese Journal of Tissue Engineering Research
基金
新疆维吾尔自治区科技厅科技支疆项目(201291173)~~
