摘要
目的观察胃癌组织中调节性T细胞以及水通道蛋白4的变化,并研究两者之间的相关性。方法纯系615小鼠60只随机分成正常组和胃癌组1、胃癌组2,各20只。正常组小鼠皮下注射0.1 mL无菌生理盐水;两个胃癌模型组小鼠皮下注射浓度为3×106/mL的0.1 mL胃癌细胞株悬液,分别于3周和4周后处死小鼠,通过流式细胞检测小鼠脾脏、淋巴结中Treg细胞的比例及数量;Western blotting检测AQP4的蛋白水平;并进一步分析其相关性。结果与正常对照组比,胃癌模型组脾脏、淋巴结中的Treg细胞比例及数量均显著增高;AQP4的表达量显著降低,并且Treg细胞的数量与AQP4的蛋白水平相关。结论调节性T细胞在胃癌中表达增加,可能与水通道蛋白4的胃癌中的表达减少有关。
[ Objective ] To explore the quantity and percentage of CD4＋CD25＋FoxP3＋ Treg cells and the expres- sion of aquaporin 4 in gastric carcinoma. [Method] Sixty mice were divided randomly into three groups and each twenty. The control group was injected with saline, and other groups were injected with gastric cancer cell line. The quantity and percentage of Treg cell was determined by flow cytometry; the expression of AQP4 was tested by West- ern blotting. [Results] The number and percentage of Treg cell in gastric carcinoma was more than that in normal group; the expression of aquaporin 4 in gastric carcinoma was significantlydecreased. The number of Treg cell corre- lated with the expression of aquaporin 4. [ Conclusion ] The increased expression of Treg cell in gastric carcinoma- may be related with the decreased expression of aquaporin 4.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第34期20-24,共5页
China Journal of Modern Medicine
