摘要
对14份发生分离性状的非整倍体无性系进行染色体水平的检测,发现原始系和变异株有丝分裂过程正常,未发生染色体水平变异。对其进行DNA甲基化分析发现不管是原始系与变异株之间,还是不同年份培养的无性系之间在CentO和Os48这2种重复序列甲基化水平没有明显的差异。此外,在染色体水平也没有发现明显全基因组甲基化的差异。
ABSTRACT： During asexual reproduction, cells divide by mitosis, so the genotype of the daughter cells should be the same as that of the original parent cell. However, somaelonal variation can occur sometimes during the process of tissue culture. In this study, among the 14 somaelonal variation lines without chromosome number variation, no abnormal mitotic chromosome behavior was detected. The DNA methylation analysis showed that they had no obvious differences in DNA methylation for the two major tandem repeats CentO and Os48 from centromerie regions and telomeric regions, respectively. In addition, no obvious methylation change was detected in whole chromosome level.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2013年第4期39-44,共6页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(30600345
31370216)
江苏省高校自然科学基金重大项目(13KJA21003)
