摘要
通过单因素试验和正交试验,确定了闪式提取松针中原化青素的最佳工艺条件,并与同流提取法比较,同时采用1,1-二苯基-2-三硝基苯肼(DPPH)法对提取所得原花青素进行抗氧化活性评价。结果显示,闪式提取松针中原花青素的最佳工艺条件为:乙醇浓度60%,料液比1:12,提取时间1.5 min,提取电压80 V;与回流提取法比较,闪式提取法在提取率、提取时间与耗电量方面优势较明显。原花青素对DPPH自由基的IC_(50)为5.44μg/ml,抗氧化效果显著优于维生素C。
The extraction process of procyanidine from pine needles by smashing tissue extraction was optimized by single factor experiment and orthogonal test. Smashing tissue extraction was compared with reflux extraction. The 1,1-diphenyl-2-picryl-hydrazyl （DPPH） method was used to identify antioxidant activity of procyanidine from pine needles. The optimal extraction conditions were as follows： ethanol concentration of 60 %, solid to liquid ratio of 1 ： 12, extraction time of 1.5 min and voltage of 80 V. Smashing tissue extraction was superior to reflux extraction in the extraction rate, extraction time and electricity consumption. The IC50 of procyanidine to DPPH was 5.44 μg/ml and its antioxidant activity was obviously higher than vitamin C.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2014年第2期120-123,共4页
Chinese Journal of Pharmaceuticals
基金
浙江省新苗人才计划(2011R465006)
关键词
松针
原花青素
闪式提取
抗氧化活性
pine needle
procyanidine
smashing tissue extraction
antioxidant activity
