摘要
目的旨在对壳聚糖纳米粒介导BMP-2基因转染骨髓基质干细胞向成骨样细胞分化的基因表达作一研究。方法根据不同浓度的壳聚糖纳米粒介导的BMP-2(pBMP-2),试验组分为CPB50组(50μg/mL pBMP-2),CPB100组(100μg/mL pBMP-2)和CPB200组(200μg/mL pBMP-2)。骨髓基质干细胞在壳聚糖纳米粒介导的BMP-2基因转染分化后,检测和分析成骨样细胞的特征性分子(碱性磷酸酶(Alkaline Phosphatase,ALP)、护骨素(Osteoprotegerin,OPG)、骨钙素(Osteocalcin,OC)、骨桥蛋白(Osteopontin,OPN))的表达水平。结果基因转染后细胞中的ALP、OPG、OC和OPN基因呈现高表达,尤其是CPB200组最高。结论壳聚糖纳米粒介导BMP-2基因转染骨髓基质干细胞后能促进细胞中成骨样细胞特征性分子的高表达。
Objective To investigate expressions of genes in osteoblast-like cells derived from bone marrow stem cells （BMSCs） transfected with chitosan nanocarrier containing plasmid-BMP-2 sequences（ CNPBs）. Methods According to different concentrations of plasmid-BMP-2 （ pBMP-2 ）, CNPBs experimental groups were divided into CPBS0 concentration group （ 50μg/mL pBMP-2）, CPB100 concentration group（ 100μg/mL pBMP-2） and CPB200 concentration group（200μg/mL pBMP-2） respectively. Expression levels of specific molecules（ Alkaline Phosphatase, ALP; Osteoprotegerin, OPG; Osteocalcin, OC ; Osteopontin, OPN） in osteoblast-like ceils were detected and analyzed. Results There were high expression levels of ALP, OPG, OC and OPN genes in osteoblast-like cells in CNPBs groups,especially in CBP200 group. Conclusions Expression levels of genes in osteoblast-like cells derived from BMSCs trans- fected with CNPBs can be promoted effectively.
出处
《口腔医学》
CAS
2013年第12期806-809,共4页
Stomatology
基金
浙江省教育厅基金(Y201018976
N20110143)
浙江省自然基金(Y2080340)
浙江省卫生厅基金(2013KYB166)
