摘要
目的建立杠板归中槲皮素和山柰素含量的HPLC测定方法。方法色谱柱：KromasilC18（5μm，250×4．6mm）；流动相：甲醇－0．4％磷酸溶液（60：40）；检测波长：360nm，柱温：30℃。结果槲皮素和山柰素的回收率分别为99．6％、100．8％；线性范围分别为0．1589-3．178μg、0．00699—0．2796μg。结论结果准确，重复性好，可用于该产品的质量控制。
Objective To establish an HPLC method for the determination of quercetin and kaempferol in Polygonum perfoliatum L.Methods HPLC was used to quantitative analysis. The Kromasil C18 column（5pm,250×4.6mm） was used,and mobile phase was composed of methanol-0.4%H3PO4/Water （ 60：40 ） . Detection wavelength was at 360 nm at 30℃. Results The quercetin and kaempferol＇s average recovery were 99.6% and 100.8%,respectively. Linear range of methods was 0.158 9-3.178μg and 0.006 99-0.279 6μg, respectively.Conclusion The result is accurate and the control of Polygonum perfoliatum L. reproducibility is good. The method can be used for quality
出处
《现代中药研究与实践》
CAS
2013年第6期69-70,共2页
Research and Practice on Chinese Medicines
关键词
杠板归
槲皮素
山柰素
含量测定
HPLC
Polygonumperfoliatum L.
quercetin
kaempferol
determination
HPLC
