摘要
目的建立铜绿假单胞菌的蛋白指纹诊断模型,为细菌的快速鉴定奠定基础。方法利用表面增强激光解析电离飞行时间质谱检测20株铜绿假单胞菌的细菌蛋白,筛选稳定表达的蛋白峰,将其数据导入自建Fingerwave软件建立铜绿假单胞菌的蛋白指纹诊断模型。收集临床分离的126株细菌,对模型的诊断能力进行评价,确定铜绿假单胞菌的鉴定阈值并应用模型对铜绿假单胞菌进行鉴定。结果建立了铜绿假单胞菌的蛋白指纹诊断模型;利用临床菌株对建立的模型进行评价,确定该模型鉴定阈值为70%,对铜绿假单胞菌鉴定结果与传统微生物学鉴定方法及分子生物学结果相比符合率为97.7%(42/43)。结论应用细菌蛋白指纹模型可快速对铜绿假单胞菌细菌进行鉴定,为细菌感染的快速诊断提供了可能性。
Objective To establish the protein fingerprint model of P. aeruginosa for rapid identification of the bacteria. Meth- ods Twenty strains of P. aeruginosa were detected by surface enhanced laser desorption ＆ ionization time-of-flight mass spectrometry （SELDI-TOF MS）. Stable expression protein peaks were screened for construction of protein fingerprint model and the data was input into self-constructed finger-wave software. One hundred and twenty-six strains of clinical isolated bacteria were collected to evaluate the diagnostic ability of the model and determinate the threshold value of P. aeruginosa. Then, the protein fingerprint model was used for identification of P. aeruginosa by protein fingerprint comparison. Results The protein fingerprint model of P. aeruginosa was construc- ted by using 19 stable expression protein peaks. Compared to the conventional approaches and molecular biology methods, the coinci-dence rate of the model was 97.7% for identification of P. aeruginosa （42/43） by using the 70% as threshold value. Conclusion P. aeruginosa can be rapidly identified by using the protein fingerprintmodel, which provides a new approach for rapid diagnosis of the bacterial infection.
出处
《实用医院临床杂志》
2014年第1期53-57,共5页
Practical Journal of Clinical Medicine
基金
四川省卫生厅科研基金资助项目(编号:120072)
关键词
铜绿假单胞菌
蛋白指纹模型
表面增强激光解析电离飞行时间质谱
P. aeruginosa
Protein fingerprint model
Surface enhanced laser desorption ＆ ionization time-of-flight massspectrometry
