摘要
采用固相萃取净化技术、固相萃取一液质联用分离和测定粮食及肉制品中苏丹红G、苏丹蓝2、苏丹红7B、苏丹红I、苏丹红II、苏丹红ⅡI、苏丹红Ⅳ、对位红、分散橙I、分散橙II、分散橙37共11种禁用工业染料。样品经正己烷提取，中性氧化铝固相萃取柱净化，采用C18柱，以10mmol／L乙酸铵-0．2％甲酸溶液（A）和甲醇-乙腈溶液（B）（7：3，V/V）为流动相，采用多反应监测（MRM）模式测定。11种染料的相关系数席均大于0．99，回收率在70％-102％之间，方法精密度小于14％（RSD，n=6），检出限在0．02～0．8μg／kg。本方法灵敏度高、操作简单高效，适合于粮食及肉制品中11种禁用工业染料的定量及确证分析。
An ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS-MS） analysis method was established for the simultaneous determination of 11 industrial dyes such as Sudan red G, Sudan blue 2, Sudan red 7B, Sudan red I, Sudan red II, Sudan red III, Sudan red IV, Para red, Disperse orange 1, Disperse orange 11, and Disperse orange 37 in foods. Samples were extracted with n-hexane, and cleaned up sequentially by neutral alumina column and solid phase extraction column. The separation was performed on an ACQUITY UPLC BEH C18 column, using a mixture of 0.2% formic acid in water with 10 mmol/L ammonium acetate and methanol-acetonitrile （7：3, V/V） as the mobile phase, and then analyzed in the multiple reaction monitoring （MRM） mode. The correlation coefficients were above 0.99, the average recoveries were 70%-102% with relative standard deviations （RSD, n = 6） lower than 14%. The limits of detection of the method were 0.02-0.8 μg/kg. This UPLC-MS-MS method is simple, effective and sensitive, and can be suitable for the determination and confirmation of residues of 11 bannedindustrial dyes in foods.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第24期174-177,共4页
Food Science
基金
北京市科技专项(Z111106055311093)
关键词
固相萃取
液质联用法
食品
工业染料
solid phase extraction
ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS-MS）
food
industrial dyes
