摘要
目的了解广东地区市场上销售的婴幼儿食品中3种食源性致病菌的污染状况。方法根据广东省人口分布情况、食品生产特点和地域分布特点选择广东省13个地区的市及县、乡镇的超市、百货、便利店和农贸市场进行采样,参照2012全国食源性致病菌监测工作手册,对婴幼儿食品(包括婴幼儿配方食品和婴幼儿谷基辅助食品)分别进行阪崎肠杆菌、蜡样芽孢杆菌和金黄色葡萄球菌的定性和定量检测。结果共采集到64个生产厂家的婴幼儿食品314份,其中婴幼儿配方奶粉92份、婴幼儿谷基辅助食品222份。314份样品中检出阪崎肠杆菌10份,检出率为3.18%,其中婴幼儿配方奶粉阳性检出率为2.17%(2/92),谷基辅助食品阳性检出率为3.60%(8/222);检出蜡样芽孢杆菌90份,检出率为28.67%,其中婴幼儿配方奶粉阳性检出率为30.43%(28/92),谷基辅助食品阳性检出率为27.93%(62/222);未检出金黄色葡萄球菌。10份阪崎肠杆菌阳性样品定量检测结果:9份<5 MPN/100g,1份>110 MPN/100g;90份蜡样芽孢杆菌阳性样品中66份采用MPN法检测,结果显示3份>103MPN/100g,41份<102MPN/100g,22份未检出,另外24份采用平板法检测,结果显示14份<102cfu/g,10份未检出。结论广东省婴幼儿食品中阪崎肠杆菌和蜡样芽孢杆菌的污染状况不容忽视,对婴幼儿的健康可能存在风险,今后应加强婴幼儿食品的监管,预防和控制婴幼儿食源性疾病的发生。
Objective To investigate contamination levels of several food pathogens in infant food in Guangdong. Methods According to the population distribution,characteristics of food production and regional distribution features of Guangdong Province,food samples were collected from supermarkets,department stores,convenience stores and farmer＇s markets in counties,towns and districts of 13 prefecturelevel cities of the province. Enterobacter sakazakii,Bacillus cereus and Staphylococcus aurea in infant food（ including infant formula milk powder and cereal based supplementary food） were detected quantitatively and qualitatively according to the Work Manual for National Food Pathogens Surveillance（ Version 2012）. Results A total of 314 samples of infant food were collected from 64 manufacturers,of which,92 were infant formula milk powder and 222 were cereal based supplementary food. Ten Enterobacter sakazakii strains were confirmed in 314 samples with a detection positive rate of 3. 18%,of which 2 strains were from infant formula milk powder（ 2. 17%） and 8 strains from cereal based supplementary food（ 3. 60%）. Ninety Bacillus cereus isolates were confirmed and the detection positive rate was 28. 67%,of which,28 strains were from infant formula milk powder（ 30. 43%） and 62 from cereal based supplementary food（ 27. 92%）. No Staphylococcus aurea strains were found. The result of quantitative determination showed that one sample was greater than 110 MPN /100g and the others were less than 5 MPN /100g for Enterobacter sakazakii,and 3 samples were greater than 103MPN /100g and 41 samples less than 102MPN /100g for Bacillus cereus. Plate count was also used to determine the number of Bacillus cereus in samples. Of 24 samples,it is found that the number of Bacillus cereus in 14 samples were less than 102CPU /100g,but no Bacillus cereus was observed in other 10 samples. Conclusion The contamination of the Enterobacter sakazakii and Bacillus cereus in infant food should not be ignored. They were potential risks for infant health. The supervision of pathogenic microorganisms in infant food and the surveillance of the related foodborne diseases should be strengthened.
出处
《华南预防医学》
2013年第6期17-19,23,共4页
South China Journal of Preventive Medicine
