摘要
以花生红衣为原料,制备A型和B型原花青素低聚体。通过体积分数70%乙醇提取并采用AB-8大孔树脂纯化得到高纯度混合物-花生红衣原花青素(PSP),反相高效液相色谱-质谱联用技术(RP-HPLC-MS/MS)对Toyopearl HW-40(S)凝胶色谱分离的两个级分PSP-3、PSP-5进行分离鉴定。结果表明:PSP的纯度大于98.0%,其得率为9.8%。PSP-3由2种B型原花青素二聚体组成,PSP-5由4种A型原花青素二聚体组成。
A-Type and B-type oligomeric proanthocyanidins were prepared from the 70% ethanol extract of peanut skin by purification on AB-8 macroporous adsorption resin and two fractions PSP-3 and PSP-5 were separated and identified by reversed phase high-performance liquid chromatography-tandem mass spectrometry （RP-HPLC-MS/MS）. Peanut skin procyanidins （PSP） with a purity of over 98.0% and a recovery of 9.8% were obtained after the purification. PSP-3 was composed of 2 B-type procyanidolic dimers and PSP-5 was composed of 4 A-type procyanidolic dimers.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第23期142-146,共5页
Food Science
