摘要
目的:检测健康人群和结核患者外周血Th17细胞和调节性T细胞(Treg细胞)(CD4+CD25+FoxP3+)在CD4+T细胞中的表达率,分析Th17/Treg细胞免疫失衡在结核病发病机制中的作用。方法:用流式细胞分析技术检测健康组人群、空洞型结核病人组、浸润性肺结核病人组和结核性胸膜炎病人组的Th17细胞和Treg细胞的表达率,用SPSS17.0统计软件分析健康人和结核病人外周血Th17细胞和Treg细胞变化,并分析Th17细胞变化和Treg细胞变化的相关性。结果:空洞型肺结核病人、浸润性肺结核病人、结核性胸膜炎病人外周血Th17细胞显著高于健康人的Th17细胞,P<0.05,分别为0.009,0.003,0.001;空洞型肺结核病人、浸润性肺结核病人、结核性胸膜炎病人的Treg细胞显著低于健康人的Treg细胞,P<0.05,分别为0.016,0.000,0.000;三组病人外周血的Th17细胞之间以及Treg细胞之间没有显著差异,P值均大于0.05;四组人群之间的Th17细胞和Treg细胞的相关性分析结果表明:结核性胸膜炎病人的Th17细胞的变化和Treg细胞变化显著负相关(r=-0.684,P=0.000),其他三组人群的Th17细胞的变化和Treg细胞变化相关性不强(P>0.05),但也都显示出了负相关趋势,相关系数r为-0.146、-0.007、-0.259;健康对照组Th17/Treg的比值显著低于三组结核病人Th17/Treg比值,P值均小于0.05,三组结核病人之间的Th17/Treg比值差异不明显,P值均大于0.05。结论:Th17细胞和Treg细胞比例失衡在结核病的发病中可能起到了重要作用。
Objective： To detect the ratios of helper type 17 T cells（ interleukin-17＋CD4＋T cells） and regulatory T cells（ T CD4＋CD25＋FoxP3＋） in peripheral blood from healthy people and patients with tuberculosis and investigate immunopathological mechanism of tuberculosis. Methods： Flow cytometry（ FCM） was used to analyze the ratios of Th17 cells and Treg cells in peripheral blood from healthy people and Cavitary pulmonary tuberculosis patients,Infiltrative pulmonary tuberculosis and Tuberculous pleurisy patients,and single factor analysis of varivance was used to analyze the varieties of Th17 cells and Treg cells,and pearson＇s linear analysis was used for the correlation between Th17 cells and Treg cells by SPSS17. 0 statistics software. Results： Th17 cells in peripheral blood from patients in Cavitary pulmonary tuberculosis group and Infiltrative pulmonary tuberculosis group and Tuberculous pleurisy group were striking higher than that from healthy people,P values were respectively 0. 009,0. 003,0. 001,but Treg cells in peripheral blood from three tuberculosis groups were lower than that from healthy group,P values were respectively 0. 016,0. 000,0. 000. There was no difference between Th17 cells and Treg cells from three tuberculosis groups（ P 0. 05）. Correlation between Th17 cells and Treg cells from healthy group and three tuberculosis groups showed that it was negative correlation（ r =- 0. 684,P = 0. 000） between increased Th17 cells and reduced Treg cells from Tuberculous pleurisy group,there was not negative correlation between Th17 cells and Treg cells from healthy group and Cavitary pulmonary tuberculosis group and Infiltrative pulmonary tuberculosis group（ P 0. 05）,but negative correlation trend could be saw about the varieties（ r =- 0. 146,- 0. 007,- 0. 259）. The ratios of Th17 cells / Treg cells from three tuberculosis groups were striking higher than from healty group（ P〉 0. 05）,but there was no difference in the ratios of Th17 cells /Treg cells among three tuberculosis groups（ P 〉0. 05）. Conclusion： The immune imbalance between Th17 cells and Treg cells may be play an important role in immunopathologicall mechanism in the development of tuberculosis.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第11期1181-1185,共5页
Chinese Journal of Immunology
