摘要
目的:研究金荞麦片对脾虚湿热证肠易激综合征腹泻型(diarrhea-predominant irritable bowel syndrome,IBS-D)大鼠免疫炎症和内脏高敏感性的影响.方法:选取SPF级新生SD大鼠,采用高乳糖饲料+慢性束缚+夹尾刺激诱导IBS-D大鼠模型.造模成功后,将实验动将物随机分为正常对照组(A组)、模型对照组(B组)、金荞麦片高剂量组(C组)、中剂量组(D组)、低剂量组(E组)、谷参肠安组(F组),金荞麦片组分别给予高剂量[6 g/(kg·d)]、中剂量[3 g/(kg·d)]和低剂量[1.5 g/(kg·d)]中药灌胃,谷参肠安组给予谷参肠安2 g/(kg·d),正常组和模型组分别给予等容积生理盐水灌胃,1次/d.灌胃4 wk后,行直肠内球囊扩张实验(colorectal distention,CRD),观察腹肌收缩反射(abdominal withdrawal reflex,AWR),评估大鼠结肠转运和感觉功能,放射免疫法检测血清肿瘤坏死因子-(tumor necrosis factor-,TNF-)、白介素-17(interleukin 17,IL-17)、IL-10、P物质(substance P,SP)和降钙素基因相关肽(calcitonin gene-related peptide,CGRP)含量,荧光定量PCR检测肠组织5-羟色胺(5-hydroxytryptamine,5-HT)、5-羟色胺3受体(5-hydroxytryptamine 3 receptor,5-HT3R)、神经激肽受体-1(neurokinin receptor 1,NK1R)和细胞间黏附分子(intracellular adhesion molecule-1,ICAM-1)mRNA表达水平.结果:与模型组比较,金荞麦片各剂量组大鼠结肠转运功能及内脏高敏感性明显降低(P<0.05或0.01),大鼠血清TNF-(2.81 ng/L±0.67 ng/L,2.76 ng/L±0.31 ng/L,2.91 ng/L±0.77 ng/L vs 3.77 ng/L±0.44 ng/L)、IL-17(2.01 ng/L±0.76 ng/L,2.14 ng/L±0.29ng/L,1.98 ng/L±0.11 ng/L vs 2.47 ng/L±0.33 ng/L)及SP(38.22 pg/mL±3.15 pg/mL,37.55 pg/mL±2.63 pg/mL,42.01 pg/mL±3.79pg/mL vs 43.19 pg/mL±4.28 pg/mL)含量均较模型组降低.IL-10(137.25 ng/L±6.2 ng/L,135.90 ng/L±4.4 ng/L,121.70 ng/L±5.11 ng/L vs 82.09 ng/L±5.33 ng/L)和CGRP(155.09 ng/L±4.38 ng/L,142.02 ng/L±2.44 ng/L,158.25ng/L±4.02 ng/L vs 107.31 ng/L±3.96 ng/L)明显升高(P<0.05或0.01).肠组织5-HT(0.74±0.43,0.97±0.91,0.66±0.29 vs 1.27±0.45)、5-HT3R(1.01±0.35,1.14±0.76,1.47±0.65 vs1.61±0.03)、NK1R(1.07±0.58,0.98±0.67,0.88±0.79 vs 1.47±0.94)和ICAM-1(0.81±0.55,0.96±0.19,0.72±0.93 vs 1.33±0.26)mRNA表达水平下调(P<0.05或0.01),尤以金荞麦片高剂量组为优.结论:金荞麦片可降低IBS-D大鼠内脏高敏感性,有效抑制免疫炎症反应,多靶点、多层次地参与调节胃肠动力.金荞麦片对实验性IBS-D具有量效依赖作用.
AIM： To determine the influence of Fagopyrum cymosum tablets on immune inflammation and visceral hypersensitivity in a rat model of diarrhea- predominant irritable bowel syndrome （IBS）.METHODS： IBS was induced in neonatal rats by feeding a high lactose diet, chronic ban- daged-stress and inserting the tail. Rats were randomly divided into six groups： a normal control group （group A）, a model group （group B）, high-, medium- and low-dose Fagopyrum cymosum groups （group C, D and E）, and a Gushen Chang＇an group （group F）. Groups C, D and E received oral administration of 6, 3 and 1.5 g/（kg·d） of Fagopyrum cymosum tablets, respectively, once every day for four weeks; group F received oral administration of 2 g/（kg·d） of Gushen Chang＇an; Groups A and B received oral administration of equal volume of normal saline. The colon motion, abdominal withdrawal reflex （AWR） of colorec- tal distention and abdominal electrical activ- ity were observed. Radioimmunoassay was used to detect serum levels of tumor necrosis factor-α （TNF-α）, interleukin 17 （lL-17）, IL-10, substance P （SP） and calcitonin gene-related peptide （CGRP）. Fluorescent quantitative PCR was used to detect the expression of 5-hydroxy- tryptamine （5-HT）, 5-hydroxytryptamine 3 re- ceptor （5-HT3R）, neurokinin receptor 1 （NKIR） and intracellular adhesion molecule-1 （ICAM-1） mRNAs in the intestinal tissue. RESULTS： Fagopyrum cymosum could de- crease colon motion and visceral hypersensitiv- ity in IBS rats （P 〈 0.05 or 0.01）. In the Fagopy- rum cymosum treatment groups, serum levels of TNF-α（2.81 ng/L ± 0.67 ng/L, 2.76 ng/L ± 0.31 ng/L, 2.91 ng/L ± 0.77 ng/L vs 3.77 ng/L ± 0.44 ng/L）, IL-17 （2.01 ng/L ± 0.76 rig/L, 2.14 ng/L ± 0.29 ng/L, 1.98 ng/L ± 0.11 ng/L vs 2.47 ng/L ± 0.33 ng/L） and SP （38.22 pg/mL ± 3.15 pg/mL, 37.55 pg/mL ± 2.63 pg/mL, 42.01 pg/mL ± 3.79 pg/mL vs 43.19 pg/mL ± 4.28 pg/mL） were significantly lower, and those of IL-10 （137.25 ng/L ± 6.2 ng/L, 135.90 ng/L ± 4.4 ng/L, 121.70 ng/L ± 5.11 ng/L vs 82.09 ng/L ± 5.33 ng/L） and CGRP （155.09 ng/L ± 4.38 ng/L, 142.02 ng/L ± 2.44 ng/L, 158.25 ng/L ± 4.02 ng/L vs 107.31 ng/L ± 3.96 ng/L） were signifi- cantly higher than those in the model group（all P 〈 0.05 or 0.01）. The mRNA expression of 5-HT （0.74 ± 0.43, 0.97 ± 0.91, 0.66 ± 0.29 vs 1.27 ± 0.45）, 5-HT3R （1.01 ± 0.35, 1.14 ± 0.76, 1.47 ± 0.65 vs 1.61 ± 0.03）, NKIR （1.07 ± 0.58, 0.98 ± 0.67, 0.88 ± 0.79 vs 1.47 ± 0.94） and ICAM-1 （0.81 ± 0.55, 0.96 ± 0.19, 0.72 ± 0.93 vs 1.33 ± 0.26） in the intestinal tissue was down-regulated after treatment with Fagopyrum cymosum tablets （P 〈 0.05 or 0.01）, especially in the high-dose group. CONCLUSION： Fagopyrum cymosum tablets can reduce visceral hypersensitivity and inhibit inflammatory immune response in IBS-D rats in a concentration-dependent manner.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第32期3543-3549,共7页
World Chinese Journal of Digestology
基金
上海市闵行区科委基金资助项目
No.2011MHZ64~~
关键词
金荞麦片
肠易激综合征
免疫炎症
内脏高敏感性
Fagopyrum cymosum tablets
Irritablebowel syndrome
Immune inflammation
Visceralhypersensitivity
