摘要
目的:探讨蛇床子素(Osthole)在大鼠肠缺血再灌注肺损伤(IIRI)中的保护机制。方法:健康雄性SD大鼠40只随机分为假手术组(sham组)、缺血再灌注组(I/R)和两个不同剂量给药组(Ost),其中缺血再灌注组和给药组采用夹闭大鼠肠系膜上动脉60 min后松开动脉夹形成缺血再灌注模型。各给药组于缺血再灌注30min后分别给予5 mg/kg、25 mg/kg蛇床子素腹腔注射治疗。各实验组动物分别采用HE染色、肺组织水肿(W/D)比值、ELISA检测和免疫组化评价蛇床子素在缺血再灌注肺损伤后的肺保护机制。结果:缺血再灌注30 min后,给药组大鼠HE,W/D、IL-1β和TNF-α结果均明显优于对照组(P<0.05),并在25 mg/kg剂量时表现出最大保护效果,同时Caspase-3蛋白表达水平也显著降低。结论:蛇床子素在大鼠肠缺血再灌注肺损伤后有一定的肺保护作用。
Objective： To investigate the lung protection of osthole against intestinal ischemia-reperfusion lung injury in rats. Methods： A total of 40 SD rats were divided randomly into 4 groups： sham operated group （Sham）; I/R group （I/R）; Osthole 5 mg / kg group （Ost5） and osthole 25 mg / kg group （Ost25）. The animals in I/R group and osthole groups were exposed to the IIRI rat model. After 60 min of occlusion and 30min ofreperfusion, the rats in osthole groups were treated with 5 mg / kg, and 25 mg / kg osthole respectively. 30 min after the reperfusion, the pathological changes by hematoxylin and eosin （HE） dyeing were observed microscopically, wet, dry （W/D） ratio, IL-1β and TNF-α of lung tissues were examined for every groups. The expression of Caspase-3 protein in the lung tissue of every group was detected by immunohistochemistry. Osthole groups were better than that of I/R group （P〈0.05）. Results： Both the histopathologieal changes, W/D, IL-1β and TNF-α ofosthole groups were better than that of I/R group （P〈0.05）. Osthole could reduce the elevated level of caspase-3 protein induced by the ischemia and reperfusion. Conclusion： Osthole exerts protective effect against intestinal ischemia-reperfusion lung injury in rats.
出处
《现代生物医学进展》
CAS
2013年第28期5401-5404,5417,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81070062)
