摘要
本研究通过对原花青素的结构分析,选取香草醛法、铁盐催化比色法两种方法对葡萄籽中的原花青素进行了检测对比。结果表明,两种方法均能稳定、有效地对原花青素进行检测。香草醛法的检测结果数值较低,且线性范围较窄,适宜用于检测黄烷-3-醇单体、低聚体酚类化合物含量;铁盐催化比色法线性范围较宽,适宜用于黄烷-3-醇不同聚合度范围的酚类化合物总量检测。
The vanillin assay method and ferric ion catalytic colorimetric method had been chosen to detect the procyanidins content on grape seed. The results showed that both of the detection methods were stable and effective. The datum from Vanillin method indicated lower values and narrower linear range than from the ferric ion catalytic colorimetric method. Vanillin method suited to detect the content of flavan- 3-alcohol monomer and oligomeric phenolic compounds; The Ferric ion catalytic colorimetric method had a wider linear range, and was suitable for the detection of the total content of flavan-3-alcohol which has a different polymerization degree.
出处
《中外葡萄与葡萄酒》
2013年第6期18-20,共3页
Sino-Overseas Grapevine & Wine
基金
山东省科技发展计划项目:葡萄籽生物活性成分分离技术集成及产业化研究(2012G0022114)
关键词
葡萄籽
原花青素
香草醛法
铁盐催化比色法
grape seed
procyanidins
vanillin assay method
ferric ion catalytic colorimetric
