摘要
目的 :为了获得更多人参DNA指纹信息 ,寻找鉴定 5个农家类型DNA特征指纹的线索 ,更有效的比较农家类型之间的遗传差异。方法 :采用扩增酶切片段多态性 (AFLP)方法研究 5种农家类型。结果和结论 :5种人参农家类型多态位点仅 4 6 % ,说明各农家类型之间的遗传差异性很小 ,但长脖的AFLP有相对较高的多态性 ,说明长脖类型内部有更多的杂合态个体 ,更接近野生人参。
Objective:To obtain more information on DNA fingerprintings of five land races of Chinese ginseng, namely, Damaya (DMY), Changbo (CB), Yuanbangyuanlu (YBYL) and Huangguo (HG). Mothods:The five land races were detected by amplified restriction fragment polymorphism (AFLP) markers with 11 combined primers (M2,M3,M16,M20,M53,M56,M57,M68,M69,M72,M84 in Mse I).Result and Conclusion:Only 4.6% polymorphic sites was found. It was further verified that only a little diversity existed amoung the land races. The polymorphic sites of CB were much more than those of the others, which suggests that there are more heterozygotes in CB populations, and it is closer to wild ginseng than the others. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2000年第12期707-710,共4页
China Journal of Chinese Materia Medica
关键词
人参
农家类型
AFLP
指纹
Panax ginseng
land races
AFLP
fingerpint
