摘要
目的 研究一种神经营养物质CBD和尼莫地平（Nimodipine，Nim）联合应用对培养大鼠皮层神经元损伤的保护作用。方法 分别采用谷氨酸化学或机械划痕创伤性损伤培养大鼠皮层神经元，根据培养细胞台盼蓝活力计数和培养上清乳酸脱氢酶（LDH）检测评估损伤程度。结果 CBD和Nim单独应用时，对谷氨酸和创伤介导的培养皮层神经元损伤有中等程度的保护作用，与单独损伤未加保护性药物组相比较有显著性差异（P<0.01）。CBD和Nim联合会用时，保护作用比单独应用明显增强（P<0.01）。结论 CBD和Nim联合应用有明显的协同作用。
Objective To investigate the protective effects of CBD and Nimodipine against injuries secondary to glutamate-inuced or traumatic injury in rat cortical neurons in vitro. Methods Mix-cultured cortical neurons of SD rat were subjected to either glutamate injury or mechanical damage. The degree of injury were detected by cell count of trypan blue staining and measurement of lactate dehydrogenase (LDH) activity in the culture medium. Results When combined treatment with CBD and Nimodipine was used separately, moderate protective effects against the injury of cultured cortical neurons induced by glutamate or trauma were observed, which was obvious when compared with the injured group without treatment (P<0.01). When combined treatment with CBD and Nimodipine was administered, strong protective effect were observed than that with treatment using CBD or Nimodipine alone (P<0.01). Conclusion It is suggested that CBD combined with Nimodipine are synergetic. Combined use of different excitotoxic antagonists can be fruitful in the therapeutic intervention of secondary traumatic damage.
出处
《第一军医大学学报》
CSCD
2000年第6期523-526,共4页
Journal of First Military Medical University
关键词
创伤性脑损伤
神经营养因子
神经元
尼莫地平
traumatic brain injury
glutamate
neurotrophic factor
neuron Cultured
