摘要
目的：完成紫锥菊口服液质量研究与评价.方法：采用硅胶GF254预制板,以乙酸乙酯-甲酸-水（6∶0.5∶1）的上层溶液为展开剂,进行TLC定性鉴别；以Agilent Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.3％磷酸水溶液梯度洗脱,流速1.2 mL·min-1,检测波长330 nm,柱温35℃,进样量10μL,进行HPLC含量测定；同时对指标pH、相对密度进行质量研究.结果：紫锥菊口服液有良好的薄层鉴别特征；有效成分菊苣酸含量在0.004 ～0.128 g·L-1,进样量与其色谱峰峰面积呈良好的线性关系（r =0.9999）,平均加样回收率101.13％,RSD 1.41％.结论：TLC定性鉴别,操作简便,重复性好.HPLC含量测定方法简便、快速、准确.所建立的方法可用于柴锥菊口服液质量控制.
Objective：To establish the quality standard and evaluation of Echinacea Purpurea oral liquid.Method：TLC test was based on silica gel plate with the upper solution of ethyl acetate-acetic acid-water （6∶0.5∶1） as mobile phase.The analytical column was Agilent Eclipse XDB-C18 （4.6 mm ×250 mm,5 μm） with acetonitrile and 0.3％ phosphoric acid as mobile phase in gradient mode; the flow rate was 1.2 mL ·min-1 ;the detecting wavelength was set at 330 nm; the column temperature was set at 35 ℃ and the injection volume was 10 μL.At the same time,pH and relative density were determined.Result：Characteristic of Echinacea Purpurea oral liquid was clear.The linear range of cichoric acid was 0.004-0.128 g ·L-1 （r =0.9999）,and the average recovery was 101.13％ with RSD 1.41％.Conclusion：The method to identify cichoric acid is simple and has a great repeatability.The method to detemine the content of cichoric acid in Echinacea Purpurea oral liquid is simple,accurate with a good reproducibility.So the TLC and HPLC methods can be used for the quality of the oral liquid.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第20期65-67,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"十二五"国家科技支撑计划项目(2011BAD44B01)
