摘要
目的 检测胃癌组织中人类表皮生长因子受体2（HER2）基因扩增与蛋白表达结果的一致性.方法 收集2010年至2012年120例胃癌患者的胃癌组织标本,其中100份为手术标本,20份为胃镜活组织检查标本.应用免疫组织化学（IHC）方法检测120份标本的HER2蛋白表达情况.根据IHC检测结果,选取IHC切片中HER2阳性部位制作成组织芯片进行荧光原位杂交（FISH）,检测HER2的基因扩增情况.对IHC检测灶性（＋＋＋）（≤10％的肿瘤细胞强染色）的标本进行不同着色强度部位对比FISH检测.统计学处理采用Kappa检验.结果 120份胃癌组织中,IHC检测显示77份为不同强度阳性染色,其中16份为（＋＋＋）,6份为灶性（＋＋＋）,37份为（＋＋）,18份为（＋）,IHC检测HER2蛋白表达阳性率为18.3％（22/120）.FISH检测显示41份阳性,HER2基因扩增率为34.2％,其中21份IHC检测为（＋＋）,15份为（＋＋＋）,5份为灶性（＋＋＋）.IHC与FISH联合检测HER2阳性率为35.0％（42/120）.IHC与FISH检测的符合率为91.6％（76/83）.Kappa系数为0.960（P＜0.01）.对5份FISH阳性且IHC检测为灶性（＋＋＋）标本的不同着色强度部位进行对比FISH检测,发现全部扩增.结论 组织芯片技术与IHC技术在胃癌组织中检出HER2具有一致性,并可提高HER2的检出率.
Objective To investigate the consistency of gene amplification and its expression at protein level of human epidermal growth factor receptor 2 （HER2） in gastric carcinoma.Methods From 2010 to 2012,120 gastric cancer specimens of patients with gastric cancer were collected,of which 100 were surgical specimens and 20 were specimens from biopsy under gastroscope.The protein expression of HER2 in 120 specimens was detected by immunohistochemistry （IHC）.According to the results of IHC,the positive parts of HER2 expression of IHC slices were developed into tissue microarrays for fluorescence in situ hybridization （FISH） to test the gene amplification of HER2.The different parts with different color intensity of focal （＋ ＋ ＋） （≤ 10% tumor cell strongly staining） specimens detected by IHC detection were compared with the results of FISH.Kappa test was performed for statistical analysis.Results Among 120 gastric cancer specimens,the results of IHC indicated that 77 specimens were positive with different staining intensity including 16 strong positive （＋＋＋）,six focal positive （＋＋＋）,37 moderate positive （＋＋） and 18 weak positive （＋）.The positive rate of HER2 protein expression detected by IHC was 18.3% （22/120）.The results of FISH showed 41 specimens were positive and the rate of gene amplification was 34.2%.Among which,21 were moderate positive （＋＋） detected by IHC,15 were strong positive （＋ ＋ ＋） and five were focal positive （＋ ＋＋）.The positive rate of HER2 was 35.0% （42/120） with IHC and FISH combined detection.The consistent rate of IHC and FISH was 91.6 % （76/83）.Kappa coefficient was 0.960 （P〈0.01）.In five positive specimens detected by FISH and which were focal positive （＋ ＋ ＋） by IHC,the different parts with different color intensity were compared with the results of FISH and gene amplification was found in all specimens.Conclusion Tissue microarray technology is consistent with IHC in HER2 detection in gastric cancer specimens and could help to improve the detection rate.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2013年第10期680-683,共4页
Chinese Journal of Digestion
关键词
胃肿瘤
受体
表皮生长因子
免疫组织化学
原位杂交
荧光
基因扩增
Stomach neoplasms
Receptor, epidermal growth factor
Immunohistochemistry
In situ hybridization, fluorescence
Gene amplification
