摘要
培育多抗性基因聚合系可为抗性育种提供重要的材料基础。通过抗源与不同恢复系和保持系进行杂交、多代回交和标记辅助选择,聚合优良的抗稻白叶枯病和稻褐飞虱基因研究,分别获得了基于不同恢复系和保持系遗传背景的抗稻白叶枯病双基因Xa21Xa23聚合系4个,这些抗性聚合系对白叶枯病的表现为高抗;分别获得抗稻白叶枯病和稻褐飞虱的抗性基因Xa21Xa23Bph24(t)聚合系2个和Xa23 Bph24(t)聚合系4个,双抗性聚合系对稻白叶枯病达到高抗水平,对稻褐飞虱的抗性接近高抗水平。结果表明,即使在Xa23表达了高水平抗性的情况下,聚合双基因Xa21Xa23仍可以在一定程度上提高聚合系对稻白叶枯病的抗性水平;此外,聚合系的两类抗性基因的抗性表达相互之间没有影响。抗性基因聚合系的培育提高了恢复系和保持系的育种价值,同时也为进一步聚合更多的重要抗性基因、培育多抗性水稻品种打下了坚实基础。
Development of resistance gene-pyramided lines provides material bases for resistance breeding in rice.In present study,crosses were made between resistant sources and restorer and maintainer lines,and the cross progenies were subjected to backcross and marker-assisted selection for several generations to accumulate superior bacterial blight （BB） and brown planthopper （BPH） resistance genes.Four twogene Xa21Xa23-pyramided lines separately based with different restorer and maintainer genetic backgrounds were developed for single BB resistance and these lines showed high level of resistance to the BB disease.Two tri-gene Xa21Xa23 Bph24（t）-and 4 two-gene Xa23 Bph24（t）-pyramided lines were developed for both BB and BPH resistance and they presented high level resistance to the BB and near high level resistance to the BPH.The study results indicated that pyramiding of two resistance genes,Xa21Xa23,to the BB could still promote resist ance level to some extent even under the circumstance of high resistance expression of the gene Xa23.And the results also indicated that the resistance expression of BB and BPH did not affect each other in their pyramided lines.In addition to offering the potential for release as cultivars,the pyramided lines will serve as useful donors of gene （s） for BB and BPH to develop multiple resistance varieties in future rice breeding programmes.
出处
《西南农业学报》
CSCD
北大核心
2013年第5期1852-1857,共6页
Southwest China Journal of Agricultural Sciences
基金
广西科学研究与技术开发计划项目(桂科攻10100005-7,桂科攻1123001-3B)
广西农业科学院基本业务费(201005基)
关键词
稻白叶枯病
稻褐飞虱
抗性育种
抗性基因聚合系
水稻
Bacterial blight （BB）
Brown planthopper （BPH）
Resistance breeding
Resistance gene-pyramided lines
Rice
