摘要
为了合理利用稀土微肥及探索镧对小麦抗盐性影响的作用机制,采用溶液培养法,研究镧(La3+)对盐胁迫下小麦种子萌发特性及幼苗活性氧代谢的影响。结果表明:9g·L-1 NaCl盐胁迫下小麦种子萌发被显著抑制,其发芽率、发芽势、发芽指数、根数、根长和芽长降低;小麦幼苗O-2产生速率、H2O2含量和膜损伤产物MDA含量增加,活性氧清除系统(SOD、CAT、POD)活性增强。用9g·L-1 NaCl与0.5~5mg·L-1镧溶液协同处理时,小麦发芽率提高,芽长增加;与0.5~10mg·L-1镧溶液协同处理时,小麦幼苗O-2产生速率和H2O2含量有所降低,MDA累积减少,SOD和CAT活性有所提高,但POD活性降低。说明较低质量浓度(0.5~10mg·L-1)的硝酸镧溶液能够缓解盐胁迫诱导的过氧化伤害,具有促进盐胁迫下小麦种子萌发和幼苗生长的作用。
The effects of lanthanum（La3＋） on germination characteristics and reactive oxygen species （ROS） metabolism in wheat seedlings were studied by solution culture for having made better use of rare earth elements on agriculture and explored the role of lanthanum on salt resistance in wheat. The results showed that under treatments of salt stress [ρ（NaC1） 9g . L-1] the seed germination was in- hibited significantly, germination rate, germination potential and germination index were decreased, as well as the root numbers, root length and shoot length, and O2 generation rate, H2O2 content and MDA accumulation in wheat seedlings increased, and the activities of ROS scavenging system （SOD, CAT, POD） were induced. When introduced La3＋ into the salt stress, the germination rate was im- proved and the shoot length were increased in co-treatments with ρ（NaCl） 9 g . L-1 and ρ[La（NO3）3 ]0.5-5 mg . L-1. To some extent, in co-treatments with ρ（ NaC1） 9 g . L-1 and ρ[-La（NO3 ）3] 0.5 10 mg . L-1 , O2 generation rate, H2O2 content and MDA accumulation also were decreased in wheat seedlings, and the activities of SOD and CAT were improved but POD activtity was decreased. It was suggested that the low concentrations（0.5--10 mg . L-1） of lanthanum nitrate maybe provided with the roles of having relieved the peroxide damage and improved the germination and seedling growth inwheat under salt stress .
出处
《西北农业学报》
CAS
CSCD
北大核心
2013年第10期90-95,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
四川省教育厅重点课题(11ZA096)
生物质材料教育部工程研究中心(西南科技大学)高技术重点课题(11zxbk03)
关键词
硝酸镧
盐胁迫
小麦
萌发特性
活性氧代谢
Lanthanum nitrate
Salt stress
Wheat
Germination characteristics
Reactive oxygen spe-cies metabolism
