摘要
为从微生物学角度揭示象山港内西沪港海域生态环境的健康状况,于2009年8月,2010年3月、5月、8月,2011年5月、8月采集了港底部和中东部的沉积物和水体样品,利用PCR-变性梯度凝胶电泳(Denaturing gradient gel electrophoresis,DGGE)技术分析了沉积物中细菌群落结构的时空变化,并结合冗余分析(Redundancy analysis,RDA)方法分析了细菌群落结构与水环境因子的相关性.PCR-DGGE分析表明西沪港内沉积物环境中的细菌类群主要有变形菌门、厚壁菌门、拟杆菌门、绿弯菌门、酸杆菌门等,其中变形菌门细菌种类最多;沉积物样品分析结果表明,港底沉积环境2009年8月的优势菌群并不明显,之后的5个时期优势菌群依次变为红螺菌目、假单胞菌目、交替单胞菌目、绿弯菌门、伯克氏菌目;港中东部沉积物的优势菌群变化比较明显,6个时期依次变为乳杆菌目、假单胞菌目、假单胞菌目、弧菌目、假单胞菌目、乳杆菌目.RDA分析表明活性硅酸盐、无机氮、化学需氧量是影响港底细菌群落结构的主要水环境因子;活性硅酸盐、无机氮、氨氮是影响港中东部细菌群落结构的主要水环境因子,表明西沪港沉积物细菌群落结构的变化是多种环境因子共同作用的结果.
In order to reveal the health status of the eco-environment of Xihu area in Xiangshan Bay, the sediment and water samples were collected in August 2009, March, May and August in 2010, May and August in 2011. The bacterial community structures and predominant bacterial species were investigated using PCR-Denaturing Gradient Gel Electrophoresis （DGGE）, and the correlation between the bacterial community and the main environmental influencing factors were analyzed by using redundancy analysis （RDA）. The results of PCR-DGGE showed the dominant bacterial phyla in sediment environment in Xihu area were Proteobacteria, Firmicutes, Bacteroides, Chloroflexi and Acidobacteria. In the bottom part, the dominant bacteria was not obvious in August 2009, but Lactobacillales, Pseudomonadales, Alteromonadales, Chloroftexi and Burkholderiales appeared as alternate dominant groups during the following 5 periods with time going on. For the middle-east part, Lactobaeillales, Pseudomonadales, Pseudomonadales, Vibrionales, Pseudomonadales, Lactobacillales were observed as alternate dominant bacteria during the 6 periods. The result of RDA analysis showed that the main environmental influencing factors on the bacterial community structures in the sediment of the bottom part were active silicate, dissolved inorganic nitrogen and chemical oxygen demand, but active silicate, dissolved inorganic nitrogen and ammonia for the sediment of the middle-east part, indicating that the variation of bacterial community structures are the results of the synergistic effects of several environmental factors. Fig 5, Tab 1, Ref36
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2013年第5期742-749,共8页
Chinese Journal of Applied and Environmental Biology
基金
海洋公益性行业科研专项经费项目(200805069)资助~~
