摘要
目的探讨摄糖后即时血糖升高在大鼠糖成瘾过程中的作用及机制。方法将30只大鼠随机分为空白对照组、蔗糖实验组和三氯蔗糖实验组,分别给予清水、30%蔗糖溶液、0.05%三氯蔗糖溶液(后两者甜度相近),自由取食2周后,采用盐酸纳洛酮诱导戒断反应以及对大鼠海马切片HE染色后进行形态学检查,通过对戒断症状的行为学指标评分、分析体重和血糖变化以及观察海马CA1区组织细胞的形态特征,探讨血糖对糖成瘾过程的影响。结果蔗糖实验组的戒断反应评分高于三氯蔗糖组[(13.80±2.03)vs(3.70±0.88),P<0.05]、空白对照组[(13.80±2.03)vs(3.70±0.68),P<0.05],差异有统计学意义。纳洛酮戒断4 h后,三组的体重下降率差异无统计学意义(P>0.05)。蔗糖组海马CA1区的组织疏松程度和细胞空泡的出现概率明显高于三氯蔗糖组和空白对照组,且差异有统计学意义(P<0.05)。结论摄糖后即时的血糖浓度变化能影响大鼠糖成瘾过程,该作用可能通过影响海马CA1区结构和功能来完成。
Objective To preliminarily discuss the function of blood sugar which is increased transitorily in the process of sugar addiction in rats. Methods 30 SD rats were included in this experiment, randomly divided into control group （n= 10） , sucrose group（n=10）and sucralose group（n=10）, which had ad libitum access to chow and water/30% sucrose solution/0.05% sueralose solution daily, respectively. After 2-week feeding, we induced withdrawal reactions by naloxone hydrochloride and judged influence of blood sugar on the process of sugar addiction by 4 indexes ： ethological scores, weight, blood sugar and the morphological features of hippocampus cells in CA1. Results The ethological score of sucrose group was statistically significantly higher than those of the other two groups [ （ 13.80 ±2.03 ） vs （3.70 ±0.88） vs （3.70 ±0.68）, P〈0.05 ]. Also, 4 hour after injected naloxone, sucrose group lost weight most, sucralose group second and control group last [ （2.96±0.32） vs （2.94±0.19） vs （2.47±0.23）, P〉0.05]. The hippocampus tissue looseness in CA1 and the probability of cellular vacuoles present in sucrose group were more obvious and higher than those of the other groups. Conclusion The level of blood sugar which is increased transitorily has an influence on the process of sugar addiction in rats which is probably by changing the morphology and function of CA1 in hippoeampus.
出处
《热带医学杂志》
CAS
2013年第9期1065-1067,1071,F0004,共5页
Journal of Tropical Medicine
基金
中山大学2012年学生暑期科研项目(73)
关键词
血糖
糖成瘾
戒断反应
blood sugar
sugar addiction
withdrawal reaction
