摘要
采用大孔树脂对蓝莓果渣多酚进行纯化,并采用HPLC-DAD-MS分析其多酚组成。结果表明:AB-8树脂的吸附量为24.38mg/g,解吸率为95.49%,并且达到吸附平衡与解吸平衡的时间较短,较适合蓝莓果渣多酚的纯化。最佳吸附条件为上样溶液多酚质量浓度2.6mg/mL、pH2、上样流速2mL/min;最佳洗脱条件为乙醇体积分数60%、洗脱流速2mL/min,在此条件下,蓝莓果渣多酚纯度由11.05%提高到59.29%。HPLC-DAD-MS检测结果显示,蓝莓果渣多酚主要组分为7种花色苷,分别为飞燕草色素-3-半乳糖苷、飞燕草色素-3-葡萄糖苷、矢车菊色素-3-半乳糖苷、牵牛花色素-3-半乳糖苷、牵牛花色素-3-葡萄糖苷、锦葵色素-3-半乳糖苷、锦葵色素-3-葡萄糖苷。
Polypehenols from blueberry pomace, a byproduct of blueberry juice production, were purified by macroporous resin chromatography and HPLC-DAD-MS was used to analyze the polyphenol composition of extracts. The results indicated that the absorption capacity and desorption capacity of AB-8 resin were 24.38 mg/g and 95.49%, respectively. In addition, the time for reaching the adsorption and desorption equilibrium in AB-8 resin was short, so it was the most suitable to purify blueberry pomace polyphenols. The optimum adsorption parameters of AB-8 resin were polyphenol concentration of 2.6 mg/mL, pH 2, feeding rate of 2.0 mL/min, and the optimal desorption parameters were ethanol concentration of 60% and elution rate of 2.0 mL/min. After purification, the purity of polyphenols was increased from 11.05% to 59.29%. HPLCDAD- MS analysis showed that the major components of blueberry pomace polyphenols included 7 kinds of anthocyanins such as delphindin-3-galactoside, delphindin-3-glucoside, malvidin-3-glucoside, petunidin-3-galactoside, petunidin-3- glucoside, malvidin-3-galactoside, and cyanidin-3-galactioside.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第20期1-6,共6页
Food Science
基金
国家自然科学基金青年科学基金项目(NSFC31101264)
2011年南京市科技发展计划国际联合研发项目(201101093)
关键词
蓝莓果渣多酚
大孔树脂
纯化
高效液相色谱-二极管阵列检测-质谱
blueberry pomace polyphenols
macroporous resin
purification
high-performance liquid chromatography-diode-array detection-mass spectrometry （HPLC-DAD-MS）
