摘要
柑橘油斑病作为一种普遍发生的柑橘病害，严重影响着果实的外观和商品价值。以晚熟脐橙“班菲”为材料，利用抑制差减杂交技术分别构建油斑病诱导的正反向cDNA文库。文库插入片段多在100～700bp之间，从正反向文库中各随机挑取了150个菌液PCR检测为阳性的克隆进行测序，成功获得了有效ESTs序列292条。通过BLASTx序列比对，有165条ESTs找到了55个基因的同源序列，且E值小于1.0×10-3。GO功能归类结果表明：这些差异表达的ESTs主要涉及氧化还原、抗逆防御、生物和非生物胁迫等多种相关的生物学过程，推测油斑病的形成与氧化胁迫有关。
Oleocellosis, a widely distributed citrus disease, has a significant impact on the appearance and commercial value of citrus fruits. To clarify the molecular mechanism of citrus oleocellosis, both forward and reverse cDNA suppression subtractive hybridization libraries were constructed using RNA extracted from navel oranges ‘Banfield’ with oleocellosis and healthy ones. Results showed that the length of majority of the inserts ranged from 100 to 700 bp. A total of 292 clones were successfully sequenced from 300 randomly picked positive clones, among which 165 ESTs in 55 genes were identified to share high homology with known genes with E-value 〈 1.0×10-3. GO and KEGG analysis showed that these differentially expressed genes （DEGs） were mainly involved in redox stress, defense response, and biotic or abiotic stress. We therefore speculated that oxidative stress might contribute to the formation of oleocellosis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第17期219-224,共6页
Food Science
基金
重庆市科技攻关计划项目(CSTC2009AB1107)
关键词
柑橘油斑病
抑制性差减文库
差异表达基因
氧化胁迫
实时定量PCR
citrus oleocellosis
suppression subtractive hybridization （SSH）
differentially expressed genes （DEGs）
oxidative stress
real-time PCR
