摘要
目的探讨益气活血方对胃溃疡愈合部位新血管的生成及促血管生成因子表达的作用。方法胃溃疡模型大鼠随机分为模型组、奥美拉唑组、益气活血方组、奥美拉唑加益气活血方组。HE染色观察新生血管数量,免疫组化测定胃组织中VEGF和VEGFR表达水平。结果奥美拉唑组溃疡指数与模型组相比,均有显著性下降(P<0.01);益气活血方加奥美拉唑组溃疡指数与奥美拉唑组相比有明显下降(P<0.05)。益气活血方组、益气活血方加奥美拉唑组与模型组、奥美拉唑组相比,14 d和21 d时微血管数量明显增多(P<0.05)。实验各组VEGF表达与VEGFR表达,均随胃溃疡愈合进程而增强,愈合后表达降低。模型组,直到28 d时表达增加。结论益气活血方主要通过增加胃溃疡黏膜组织中VEGF和VEGFR表达,促进新血管的生成,从而提高溃疡愈合质量,中西药合用具有协同增效作用。
Objective To study the effect of Yiqihuoxuefang （YQHXF） on rats with gastric ulcer and investigate the formation of new blood vessels and angiogenesis factor of the ulcer healing tissue. Methods SD rats induced with gastric ulcer were randomly divided as： mode group, OMLZ group, YQHXF group, YQHXF ＋ OMLZ group. Microvessels in connective tissue were observed quantitatively by hematoxylin eosin stain, and VEGF and VEGFR in the gastric ulcer tissues was examined by immunohistoehemical method. Results The index of ulcer of OMLZ group decreased significantly than that of model group（ P 〈 0.01 ）. The index of ulcer of YQHXF ＋ OMLZ group decreased significantly than that of OMLZ group （ P 〈 0.05）. After 14 or 21 days, microvessels of YQHXF group and YQHXF ＋ OMLZ group increased significantly than that of model group and OMIZ group. VEGF/VEGFR＇S expression of each group was increased. Conclusion It is indicated that YQHXF can enhance the expresion of VEGF/VEGFR and micmvessels in connective tisue increased and and improve quality of ulcer healing. Chinese medicinal materials and Western medicine combined with synergistic effect.
出处
《吉林中医药》
2013年第8期827-830,共4页
Jilin Journal of Chinese Medicine
基金
江苏省中医药局2007-2008年度中医药科研项目(第二批)(HL07105)
2007年苏州市科技发展计划(社会发展与医药)项目(SS0710)
2010年苏州市"科教兴卫"青年科技项目
