摘要
本研究构建了长期低渗胁迫诱导凡纳滨对虾肝胰腺差异表达基因的cDNA文库。在此基础上,探讨了在不同盐度处理时反向文库5个基因(血蓝蛋白、蜕皮类固醇调节蛋白、几丁质酶、胰凝乳蛋白酶1和胰蛋白酶)mRNA表达量的变化情况,以期从分子机理上了解,在长期低盐养殖条件下凡纳滨对虾的生长性能和生理状态的关系。长期低盐养殖实验结果表明,随着盐度的降低,凡纳滨对虾的末重、增重率和特定生长率显著地降低(P<0.05);盐度2处理组成活率显著低于盐度10处理组和对照组(P<0.05);与对照组相比,盐度2处理组血蓝蛋白、几丁质酶、蜕皮类固醇调节蛋白、胰蛋白酶和胰凝乳蛋白酶1mRNA表达量显著性下调,分别下降了50%、31%、91%、25%和35%;盐度10处理组血蓝蛋白、胰蛋白酶和胰凝乳蛋白酶mRNA表达量显著性下调,分别下降了31%、72%和15%;短期低盐胁迫组几丁质酶和蜕皮类固醇调节蛋白mRNA表达量上调3.07和2.47倍。结果表明,盐度降低到一定程度,可显著降低凡纳滨对虾的生长性能和成活率;5个基因表达量在胁迫24h和56d后表达量变化明显不同,说明凡纳滨对虾对长期和短期盐度胁迫采取不同的应答策略。以上结果丰富了甲壳动物环境胁迫研究的基因信息。
We determined the transcript abundance roid regulated protein, trypsin and chymotrypsin 1） change of 5 protein （hemocyanin, chitinase, ecdysteencoding genes at different salinities （2, 10 and 30） with quantitative real-time PCR （qRT-PCR） in order to decipher the relationship between growth performance and physiological status of long-term low salinity breeding of Litopenaeus vannamei. The cDNA library was constructed with RNA extracted from the hepatopancreas. Long-term low salinity breeding significantly decreased the final weight, weight gain and specific growth rate of L. vannamei （P〈0. 05）. At salinity 2, shrimp had the lowest growth and survival in comparison with those at salinity 10 and 30 （P〈0.05）. In comparison with seawater, the expression of 5 genes decreased 50%, 31%, 91%, 25% and 35%, respectively, at salinity 2; the expression of hemocyanin, trypsin and chymotrypsin 1 gene de- creased 31%, 72% and 15%, respectively, at salinity 10% while the expression of chitinase and eedyste- roid-regulated protein gene increased 3. 07 and 2. 47 fold, respectively. These findings indicated that low salinity may reduce growth performance and survival rate significantly. The significant difference in the expression of 5 genes between long-term （56 days） and short-term （24 hours） salinity stress implied the difference in responsive mechanism to salinity stress.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第9期39-46,共8页
Periodical of Ocean University of China
基金
国家自然科学基金项目(30871928)
广东省高等学校珠江学者特聘教授基金项目(GDUPS-2011)
公益性行业(农业)科研专项基金项目(201003020)资助
关键词
凡纳滨对虾
肝胰脏
盐度
胁迫
基因表达
. Litopenaeus vannamei
hepatopancreas
salinity
stress
gene expression
