摘要
以茯苓为原料,研究了茯苓多糖的响应面提取条件优化及体外免疫活性。结果表明:茯苓多糖提取最佳工艺条件为微波功率471.3W,微波提取时间8.5min,乙醇浓度82.3%,液料比为19.9,在该条件下,茯苓多糖的提取率为2.96%。茯苓多糖具有较好的刺激淋巴细胞增殖的能力,随着茯苓粗多糖浓度的增加,其刺激淋巴细胞增殖的能力也逐渐增强,当茯苓粗多糖浓度超过100μg/mL时,其刺激淋巴细胞增殖的能力反而下降。
Taking Poria coccus as material, the extraction technology of surface methodology and its immune activity was studied. The results polysaccharides from it was optimized by response showed that the optimum extraction conditions of polysaccharides from Poria coccus was microwave power 471.3 W, microwave extraction time 8. 5 min, ethanol concentration 82. 3%, solution and material ratio 19. 9, under these conditions, the polysaccharides yield could reach 2. 96%. The polysaccharides from Poria coccus had proliferative effect on leukomonocyte. The proliferative effect strengthened gradually with the concentration of polysaccharides from Poria coccus increased. When its concentration exceeded 100μg/mL,the proliferative effect decreased.
出处
《北方园艺》
CAS
北大核心
2013年第17期135-138,共4页
Northern Horticulture
关键词
茯苓
多糖
响应面法
体外免疫活性
Poria coccus
polysaccharides
response surface methodology
immune activity
