摘要
目的探讨组织蛋白酶K在大鼠磨牙再植术后不同时间段牙髓细胞内的表达分化及其分布。方法 20只8周Wistar雄性大鼠,分为4组,每组5只。单侧上颌第一磨牙脱位后即刻再植,对侧同名牙为自身对照。用原位杂交方法检测组织蛋白酶K在大鼠磨牙再植术后14天、21天和28天牙髓细胞内的mRNA表达情况。结果牙髓细胞、成牙本质细胞中组织蛋白酶K原位杂交染色阳性,表达比对照组高(P<0.05)。14天与21天和28天比较,各种细胞组织蛋白酶K表达差异有统计学意义(P<0.05),而21天和28天比较,各种细胞内的组织蛋白酶K表达差异无统计学意义(P>0.05)。结论组织蛋白酶K参与了牙再植术后牙根的吸收过程,组织蛋白酶K可能促进了破牙细胞分化成熟。
Objective To investigate the function and the expression of cathepsin K mRNA in pulp cells after teeth replantation. Methods Twenty 8-week-old Wistar male rats were select- ed. The unilateral maxillary first molar of each rat was extracted and replanted immediately, nothing was done on the other side which was as control. The rats were randomly devided into four groups and were sacrificed on day 14, day 21 and day 28 after replantation. Cathepsin K was observed in various stages through in situ hybridization. Results Cathepsin K positive sig- nals were found in pulp fibroblast, odontoblast in various root resorption which were higher than that in the control group and the expression of cathepsin K in different cells of the 14 day of pri- mary teeth was significantly different from that of the other two groups （ P 〈 0. 05 ）, but there was no obvious difference between the 21 day and 28 day of the resorption of teeth replantation （P 〉 0. 05 ）. Conclusion Cathepsin K was likely to participate in the root resorption of teeth replantation. Cathepsin K may also improve the odontoclastogenesis activation and maturation.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2013年第4期321-323,328,共4页
Journal of Harbin Medical University
基金
黑龙江省科技青年基金资助项目(QC06C077)
关键词
组织蛋白酶K
牙再植术
原位杂交
cathepsin K
teeth replantation
in situ hybridization
