摘要
以甜菜坏死黄脉病毒内蒙分离物 (BNYVVNM )总RNA为模板 ,经RT -PCR扩增 ,分别获得RNA2、RNA3和RNA4自然缺失突变体的cDNA克隆。序列分析结果表明 ,RNA2自然缺失突变体在 75kD通读蛋白编码区C端缺失 34 8个核苷酸 (缺失位置nt1488～nt1835 )。RNA3在其 2 5kD蛋白编码区内缺失 36 0个核苷酸 (缺失位置nt72 9～nt10 88)。RNA4的自然缺失区域位于 31kD蛋白编码区 ,缺失 40 2个核苷酸 (nt70 2～nt110 3) ,缺失未造成移码 ,仍可编码产生一个由 148个氨基酸组成的蛋白。BNYVV内蒙分离物上述 3个RNA组分的自然缺失突变体的缺失区域 ,与国外报道的德国G1分离物和日本S分离物的自然缺失区域非常相似。同时 ,对自然缺失的可能机制及其在病害防治上的应用进行了讨论。
Spontaneously deleted RNA2, RNA3 and RNA4 cDNA clones were obtained respectively by cloning the RT PCR products of beet necrotic yellow vein virus NM isolate from Inner Mongolia into pGEM 7Zf(+). The sequence analysis showed that the spontaneously deleted RNA2 has 348 nucleotide deletion (nt1,488 nt1,835) within its C terminal region of the 75 kD readthrough protein gene. The RNA3 has 360 nucleotide deletion(nt729 nt1,088) within its 25kD protein ORF region. The RNA4 has 402 nucleotide deletion(nt702 nt1,103) within its 31kD protein ORF region, which encoded a protein consisting of 148 amino acids. The spontaneously deleted regions in the RNAs of BNYVV NM isolate were much more similar to the German isolate G1 and Japanese isolate S. The possible mechanism of spontaneous deletion of the virus RNAs and its application for control of the disease were discussed in this paper as well.
出处
《病毒学报》
CAS
CSCD
北大核心
2000年第1期49-53,共5页
Chinese Journal of Virology
基金
国家自然科学基金!( 3 9670 0 3 5
3 9770 492 )
关键词
甜菜坏死黄脉病毒
自然缺失突变体
序列分析
beet necrotic yellow vein virus
spontaneously deleted RNAs
sequence analysis
