摘要
基于微尺度(微/纳米)功能生物界面的成像与表征,集成并发展了以原子力显微镜、环境扫描电子显微镜等纳米源头技术为主导的,具有相互协同、验证、补充的多信息、多层次联合成像、表征及微加工设备功能群,实现了活体生物界面微尺度成像与表征方法学上的突破.进而,强调"医学功能界面"的概念,针对血管、骨和肿瘤相关医学功能界面,深入开展"微尺度构建-功能-力学耦合机制"研究.在此基础上,受血管内皮细胞为载体的血流/血液/血管相互作用功能界面的启发,实现黏附可控医学功能界面的仿生设计与制备;同时在中医"补气活血"理论的指导下,开辟"生物力药理学"这一新的交叉研究领域,强调生物力学因素在药理学研究和临床诊疗活动中的重要作用,建立可作为Biomarker另一类形式的临床样品微尺度力学参数指标,并倡导将"实验台/病床"双向引导的转化医学模式实施于诊断与治疗中.
Focusing on the nanobiomedical imaging, medically functional biointerfaces and translational medicine, we have developed a functional cluster of imaging, nano-characterization, nano-fabrication and micromanipulation facilities, including two combined imaging systems based on the atomic force microscopy （AFM） and the environmental scanning electron microscopy （ESEM）, which provides a perfect platform for real-space studies on living micro/nanoscale biointerfaces at high resolution. Methodological breakthroughs in the imaging of living biointerfaces have been made as a result. Using the above techniques, our research concentrates on medically functional biointerfaces, i.e. special functional biointerfaces which are closely related to human health, disease and medical activities. The concept of ＂micro/nanoscaled topography-coupled-mechanics mechanisms （TCMM）＂ has been introduced into the study of medically functional biointerfaces, focusing specially on interfaces in blood vessels, bone and cancer tissues. Inspired by the functional biointerface among blood/vessel/blood-flowing and its related theory extracted from Chinese medicine, we put forward a novel borderline discipline--＂biomechanopharmacology＂ to underline the relationship between biomechanics and pharmacology in the biomedicine field. In view of the drawbacks of biomedicine approaches to date, we advocate for translational medicine, and emphasize the two-way relationship between the ＂bench＂ and the ＂bedside＂ in the field of medically functional biointerfaces at the micro/ nanoscale. As a result, the artificial-adhesion controllable interfaces, such as bioinspired micro/nanoscale interfaces for better blood compatibility or aptamer-mediated efficient capture and release of T lymphocytes were developed. Moreover, we also translated microscale mechanical parameters for use as ＂biomarkers＂ in clinical tests. To sum up, our researches are expected to open a new way to better understand the treatment of major diseases, such as cardiovascular diseases and cancer.
出处
《科学通报》
EI
CAS
CSCD
北大核心
2013年第24期2449-2465,共17页
Chinese Science Bulletin
基金
国家重点基础研究发展计划(2012CB933800
2005CB724700)
中国科学院重大科研装备研制项目(YZ200831
Y2005024)
国家自然科学基金重大研究计划面上项目(90709054)资助
关键词
纳米生物医学
医学功能生物界面
原子力显微镜环境
扫描电子显微镜
设备功能群
生物力药理学
转化医学
nanomedicine, medically functional biointerfaces, atomic force microscope, environmental scanning electron microscope, functional cluster into devices, biomechanopharmacology, translational medicine
