摘要
目的研究FOXP3在HR-HPV阳性宫颈上皮内瘤变组织中的表达及与宫颈黏膜HR-HPV负荷量间的关系。方法取HR-HPV阳性的宫颈上皮内瘤变(CIN)宫颈组织标本120例,其中CINⅠ、CINⅡ、CINⅢ各40例,HPV阴性的正常宫颈组织30例为对照组。HR-HPV负荷量采取HC2法检测,FOXP3采取免疫组织化学方法(Elivision二步法)检测。结果 FOXP3不仅表达于CIN宫颈间质的淋巴细胞,而且表达于CIN宫颈上皮细胞;FOXP3在CIN上皮组织中的表达水平与宫颈病变级别密切相关,而在CIN宫颈间质中的表达与病变级别无明显关系;FOXP3在CIN上皮组织和间质组织中的表达与宫颈黏膜HR-HPV负荷量均呈正相关关系(r分别是:0.601、0.386,均P<0.001)。结论转录因子FOXP3可考虑作为宫颈上皮内瘤变的预后因子。
Objective To investigate the relationship between FOXP3 expression in HPV-positive cervical intraepithelial neoplasia（CIN） tissue and HR-HPV load in cervical mucosa.Methods HR-HPV load was measured by hybrid capture Ⅱ and FOXP3 expression was detected by immunohistochemical staining in 120 HR-HPV-positive CIN tissues（40 CINⅠ tissues,40 CINⅡ tissues and 40 CINⅢ tissues） and 30 normal HPV-negative cervical tissues.Results FOXP3 expression was found not only in cervical interstitial lymphocytes,but also in cervical epithelial cells.The grade of cervical lesions was closely correlated with the levels of FOXP3 expression in epithelial tissue,but not correlated with the expression of FOXP3 in the stroma.In addition,FOXP3 expression in HPV-positive CIN epithelial tissue and stromal tissue was positively correlated with HR-HPV load in cervical mucosa（r=0.601 and 0.386,respectively;P〈0.001）.Conclusion The transcription factor FOXP3 can be considered as a prognostic factor in CIN.
出处
《南昌大学学报（医学版）》
CAS
2013年第6期46-48,共3页
Journal of Nanchang University：Medical Sciences
