摘要
建立一种共振散射光猝灭法测定粮食中微量硒的新方法。以小扁豆和贼小豆为样品,用pH3.500的B-R缓冲溶液为介质,硒(Ⅳ)与一定量的I-反应生成等物质的量I3-,进一步与阳离子染料罗丹明6G反应生成离子缔合物,引起592.2nm共振散射光的线性猝灭。结果表明:在最佳测定条件下,测定体系共振散射光的相对猝灭强度ΔI与硒在0～60μg/L质量浓度范围内有良好线性关系,方法的检出限为1.710μg/L,小扁豆和贼小豆硒含量测定的相对标准偏差(RSD)分别为3.9%、1.9%(n=7),回收率分别为95.56%、96.80%,测定结果令人满意。
A new method to determine microamounts of selenium in the seeds of Lens culinaris and Vigna minima was established using resonance scattering light quenching.In pH 3.500 B-R buffer solution,selenium（Ⅳ） reacted with a certain amount of I-to produce an equal molar amount of I3-,which further reacted with rhodamine 6G to form an ion association complex causing linear quenching of resonance scattering light at 592.2 nm.The results showed good linear relationship between resonance scattering light relative quenching intensity ΔI and Se（Ⅳ） concentration in the range of 0–60 μg/L under optimized conditions.The detection limit of the method was 1.710 μg/L.The precision（RSD） for 7 replicate measurements of selenium in the seeds of Lens culinaris and Vigna minima was 3.9% and 1.9%,respectively,and the average spiked recovery was 95.56% and 96.80%,respectively.These results were satisfactory.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第16期261-264,共4页
Food Science
基金
河南省重点学科建设基金项目(10466-X-082301)
关键词
小扁豆
贼小豆
硒
共振散射光猝灭法
lentils（Lens culinaris）
Vigna minima
selenium
resonance scattering light quenching method
