摘要
对红景天中红景天苷微波辅助乙醇提取工艺进行优化,并用高效液相色谱法检测红景天苷含量。实验先采用单因素试验分析原料粒度、微波时间、微波功率、乙醇体积分数、浸提温度、浸提时间、液料比7个因素对红景天苷提取率影响,再采用四因素三水平响应面分析法进行试验,并对各个因素的显著性和交互作用进行分析。结果表明:最优工艺条件为原料粒度40目、微波时间42s、微波功率480W、乙醇体积分数78%、提取温度72℃、浸提时间90min、液料比10:1(mL/g),此条件下红景天苷提取率为93.53%。
The process conditions for microwave-assisted extraction of salidroside from Rhodida rosea were optimized.HPLC was used to detect salidroside.The effects of seven process parameters including material particle size,microwave treatment time,microwave power,alcohol concentration,extraction temperature,extraction time and solid-liquid ratio on the extraction rate of salidroside were investigated through single factor tests.The significance and interaction effects of microwave treatment time,microwave power,ethanol concentration and extraction temperature were analyzed by response surface analysis based on a three-level central composite design.The optimal microwave-assisted extraction conditions for salidroside were achieved to be microwave treatment duration of 42 s,microwave power of 480 W,ethanol concentration of 78%,solid-liquid ratio of 1：10（g/mL） and extraction temperature of 72 ℃,extraction time of 90 min and material particle size of 40 mesh.Under these extraction conditions,the extraction rate of salidroside from Rhodida rosea was up to 93.53%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第14期147-151,共5页
Food Science
关键词
红景天苷
提取工艺
优化
响应面法
salidroside
extraction process
optimization
response surface methodology
