摘要
背景:血管内皮生长因子在促进骨质疏松性骨折愈合中发挥重要作用,而它是否影响骨密度变化还没明确。目的:观察去势大鼠血清血管内皮生长因子水平和骨密度及成骨细胞变化的相关性。方法:SD雌性大鼠40只随机数字表法均分为去势组和对照组,3个月后测大鼠全身、腰椎及股骨骨密度。大鼠ELISA试剂盒测血清中血管内皮生长因子的水平,同时两组大鼠股骨干骺端固定,脱钙,脱水、石蜡包埋、切片,苏木精-伊红染色,每切张片随意取5个视野(10×40)行股骨远侧干骺端成骨细胞计数在光学显微镜。结果与结论:去势3个月后大鼠体质量明显增加(P<0.05),去势组全身、腰椎及股骨骨密度较对照组全身、腰椎及股骨骨密度降低(P<0.05),表明骨质疏松的模型建立。而去势大鼠和对照大鼠血管内皮生长因子水平比较差异无显著性意义(P>0.05),去势组及对照组的成骨细胞数量无明显差异(P>0.05),去势组及对照组骨密度与成骨细胞数及血清血管内皮生子水平无相关性。提示去势大鼠的骨密度下降,体质量升高,而去势大鼠骨密度的降低与血清血管内皮生长因子变化可能无关。
METHODS： Forty female Sprague-Dawley rats were randomly divided into ovariectomized group and control group. After 3 months, the bone mineral density of the whole body, femur and lumbar spine was measured. Rat enzyme-linked immunosorbent assay kit was used to measure the level of serum vascular endothelial growth factor. Then, the rats in two groups received femoral metaphyseal fixation, decalcified, dehydrated, embeding in paraffin, slicing and hematoxylin-eosin staining. Each slice was free to take five fields of view （10x40） in order to count the osteoblasts of femur.distal metaphysis under optical microscope. RESULTS AND CONCLUSION： After ovariectomized for 3 months, the rats body mass was increased significantly （P 〈 0.05）, and the bone mineral density of the whole body, femur and lumbar spine in the ovariectomized group was lower than that in the control group （P 〈 0.05）, indicating the successful establishment of osteoporosis model. There was no significant difference in vascular endothelial growth factor level between the ovariectomized group and the control group （P 〉 0.05）, and the difference of the osteoblast number between ovariectomized group and control group was not significant （P 〉 0.05）. This indicated that there was no correlation between bone mineral density and the number of osteoblasts and vascular endothelial growth factor level in the ovariectomized group and the control group. These findings suggest that the bone mineral density is reduced and the body mass is increased in the ovariectomized rats, and the reduced bone mineral density of ovariectomized rats may be irrelevant with the change of serum vascular endothelial growth factor.
出处
《中国组织工程研究》
CAS
CSCD
2013年第28期5113-5119,共7页
Chinese Journal of Tissue Engineering Research
基金
山西省科技攻关项目(20100311098-2)~~
关键词
组织构建
骨组织构建
去势大鼠
骨质疏松
血管内皮生长因子
成骨细胞
骨密度
股骨干骺端
省级基金
tissue construction
bone tissue construction
ovariectomized rat
osteoporosis
vascular endothelial growth factor
osteoblasts
bone mineral density
femoral metaphysic
provincial grants-supported paper
