摘要
采用乙醇/硫酸铵双水相体系萃取茶叶中茶多酚。通过单一因素实验优化了影响茶多酚萃取率的各因素,通过正交实验确定了各因素的影响程度:即粗提液的用量>硫酸铵的用量>pH>乙醇的用量>分相温度。实验最佳提取条件为,当硫酸铵的质量分数为0.2 g/mL,乙醇的体积分数为50%,粗提液的体积分数为20%,pH为6.8,分相温度为40℃时,茶多酚的萃取率达到97.92%,分配系数为22.17。对测定样品进行加标回收试验,其回收率为98.6%～104.55%。对样品溶液进行十次平行测定测得标准偏差为0.235%。粗提液中茶多酚的浓度为0.365μg/mL。从而建立了一种从茶叶中萃取茶多酚的新方法。
Used ethanol / ammonium sulfate aqueous two-phase to extract tea polyphenols.By single factor experiment to optimize the factors affecting,the impact of various factors was determined by orthogonal experiment： the crude extract dosage the amount of ammonium sulfate pH the quantity of ethanol phase separation temperature.The optimum extraction condition： the mass fraction of ammonium sulfate is 0.2 g/mL,the volume fraction of ethanol is 50 %,the volume fraction of the crude extract liquid is 20 %,pH=6.8,phase separation temperature is 40 ℃,the extraction of tea polyphenols rate reached 97.92 %,the distribution coefficient was 22.17.Determination of the samples were spiked recovery test,the recovery rate was 98.6 %～104.55 %.For ten parallel determination,standard deviation was 0.235 % for the sample solution.The concentration of crude extracts of tea polyphenols was 0.365 μg/mL.Thus a new method was establish to extract tea polyphenol from tea.
出处
《广东化工》
CAS
2013年第14期26-28,32,共4页
Guangdong Chemical Industry
基金
南京理工大学泰州科技学院"梦兰神彩实践创新奖励基金"项目
关键词
茶多酚
吸光度
双水相萃取
乙醇
硫酸铵
tea polyphenols
absorbance
aqueous two-phase extraction
alcohol
ammonium sulfate
