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Charaterization of Citrus Hybrid "Huangguogan" through the Combination of Morphological and Molecular Markers 被引量:3

Charaterization of Citrus Hybrid "Huangguogan" through the Combination of Morphological and Molecular Markers
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摘要 Huangguogan, an obvious Citrus hybrid, is suitable for transportation and export and ripens in March or April. Because of late season, it may playa significant role in fruit market. However, its origin is still unconfirmed. The aim of this study was to clarify the possible parentage of Huangguogan via the combination of morphological and molecular markers including simple sequence repeat (SSR) and chloroplast simple sequence repeat (cpSSR). Analysis of morphological traits including leaf stalk length, phylliform index and fruit shape index indicated that Huangguogan had similarities in morphology with Sweet orange. The SSR Cluster Analysis showed that Huangguogan was clustered together with Hongju tangerine and revealed -80% genetic similarity. They illustrated a close genetic distance between Huangguogan and Hongju tangerine. In addition, the bands of2 polymorphic cpSSR were identical in Huangguoggan and Sweet orange. Consequently, it is likely that its female parentage was the sweet orange (Citrus sinensis (L.) Osbeck) and its male parentage was the tangerine (Citrus reticulata Blanco).
出处 《Journal of Life Sciences》 2013年第4期348-352,共5页 生命科学(英文版)
关键词 CITRUS natural hybrid huangguogan morphological and molecular markers. 分子标记 形态学 柑橘 遗传相似性 混合 刻划 简单重复序列 SSR
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