摘要
采用反相高效液相色谱梯度洗脱法 ,用ODS柱 ,以甲醇 醋酸 三乙胺 水为流动相 ,在 2 30nm处同时测定唐威胶囊中盐酸小檗碱及葛根素的含量。平均添加回收率 (n =5)和RSD值分别为 98 2 %和 1 6%(盐酸小檗碱 ) ;97 5%和 1 8%(葛根素 )。
A reversed phase high performance liquid chromatographic gradient elution method was developed for the determination of beberine hydrochloride and puerarin in Tangwei capsules Separation was obtained by using Hypersil ODS (150 mm×4 6 mm i.d., 5 μm)column and mobile phase of methanol and acetic acid, triethylamine water solution Detection was performed with UV detector at 230 nm The column temperature was 35 ℃ The calibration curves were linear within the range of 0 18 μg 1 66 μg for beberine hydrochloride (n=5,r=\{0 999 9\}) and 0 09 μg 0 89 μg for puerarin (n=5,r=\{0 999 8\}) The recovery and RSD of beberine hydrochloride were 98 2% and 1 6% and those of puerarin were 97 5% and 1 8%,respectively
出处
《色谱》
CAS
CSCD
北大核心
2000年第5期465-467,共3页
Chinese Journal of Chromatography
关键词
唐威胶囊
盐酸小檗碱
葛根素
糖尿病药物
HPLC
high performance liquid chromatography
Tangwei capsules
beberine hydrochloride
puerarin
gradient elution
