摘要
目的探讨microRNA-143(miR-143)对人乳腺癌MCF-7细胞侵袭、迁移和增殖的影响。方法在脂质体介导下将miR-143抑制剂(miR-143 inhibitors)转染入MCF-7细胞,以inhibitor negative control(inhibitor NC)作为阴性对照。通过MTS试剂盒检测细胞增殖能力,以Transwell侵袭实验和迁移实验分别检测细胞侵袭能力和迁移能力。结果(1)miR-143 inhibitors组与inhibitor NC组间细胞增殖活性无明显差异(P>0.05);(2)Transwell侵袭及迁移实验显示转染miR-143 inhibitors后,MCF-7细胞的侵袭及迁移能力均明显增强(P<0.05)。结论 miR-143对人乳腺癌MCF-7细胞的侵袭及迁移能力存在负性调控作用,可能成为乳腺癌治疗的潜在靶点。
Objective To investigate the effect of microRNA-143 （miR-143） on invasion, migration and proliferation po- tential of human breast cancer MCF-7 cells. Methods MCF-7 cells were transfected with miR-143 inhibitors by lipofectamine. MCF-7 cells transfected with inhibitor negative control were used as negative control（inhibitor NC）. Cell proliferation potential was evaluated by MTS kit, cell invasion potential and migrating ability were detected by transwell invasion and migration assay. Re- sults There were no significantly differences in cell growth between cells transfected with miR-143 inhibitors and inhibitor NC cells（P 〉0.05）. Transfection of miR-143 inhibitors into MCF-7 cells led to a significant increase in cell invasion and migration de- tected by transwell invasion and migration assay（P〈0.05）. Conclusion MiR-143 negatively regulates the invasion and migration potential of human breast cancer MCF-7 cells and may be a potential target for breast cancer treatment.
出处
《浙江医学》
CAS
2013年第11期1049-1051,共3页
Zhejiang Medical Journal
基金
浙江省医药卫生科技计划项目(2012KYB230)
